Reversible Increase in Resistance of A-431 Carcinoma Cells to TRAIL-Induced Apoptosis in Confluent Cultures Corresponds to a Decrease in Expression of DR4 and DR5 Receptors

被引：0

作者：

Fadeev, R. S. ^{[1
,2
]}

Dolgikh, N. V. ^{[1
]}

Chekanov, A. V. ^{[1
]}

Senotov, A. S. ^{[1
]}

Krasnov, K. S. ^{[1
,2
]}

Kobyakova, M. I. ^{[1
]}

Lomovskaya, Ya. V. ^{[1
]}

Fadeeva, I. S. ^{[1
,2
]}

Akatov, V. S. ^{[1
,2
]}

机构：

[1] Russian Acad Sci, Inst Theoret & Expt Biophys, Pushchino 142290, Moscow Oblast, Russia

[2] Puschino State Nat Sci Inst, Pushchino 142290, Moscow Oblast, Russia

来源：

BIOLOGICHESKIE MEMBRANY | 2023年 / 40卷 / 02期

关键词：

human carcinoma cells; TRAIL-induced apoptosis; confluent culture; TRAIL resistance; TRAIL receptors; LEUKEMIA-CELLS; ACTIVATION; MECHANISMS;

D O I：

10.31857/S0233475523020020

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

TRAIL (TNF alpha Related Apoptosis Inducing Ligand) cytokine is of great interest for the development of targeted antitumor drugs. We have previously found a reversible increase in tumour cell resistance to TRAIL-induced apoptosis in confluent cultures. In this work we show that increase in resistance of A-431 cells to TRAIL-induced death in confluent culture is associated with reduced expression of pro-apoptotic receptors DR4 and DR5 with absence of anti-apoptotic receptors DcR1 and DcR2 on cell surface. Decreased representation of DR4 and DR5 receptors on the cell surface is accompanied by a lack of activation of the proapoptotic protein Bid, effector caspase 3 under the action of recombinant protein izTRAIL, which leads to an increase in TRAIL resistance. Our results indicate that reversible increase in resistance of human carcinoma A-431 cells to TRAIL-induced apoptosis in confluent cultures is caused by decrease in expression of DR4 and DR5 receptors on cell surface.

引用

页码：105 / 111

页数：7