Selective visualization of nitric oxide in living cells and tissue slices using a mitochondria-targetable fluorescent probe

被引:8
作者
Tang, Changquan [1 ]
Wang, Mingxue [2 ,3 ]
Wu, Changfeng [2 ]
Zheng, Qingdong [1 ]
机构
[1] Chinese Acad Sci, Fujian Inst Res Struct Matter, State Key Lab Struct Chem, 155 Yangqiao West Rd, Fuzhou 350002, Peoples R China
[2] Southern Univ Sci & Technol, Dept Biomed Engn, Shenzhen 518055, Peoples R China
[3] City Univ Hong Kong, Dept Biomed Engn, Kowloon, Hong Kong 999077, Peoples R China
基金
中国国家自然科学基金;
关键词
Nitric oxide; Probe; Mitochondrial; Fluorescent; Two-photon imaging; DEHYDROASCORBIC ACID; AUTOPHAGY; EFFICIENT; NO;
D O I
10.1016/j.dyepig.2023.111443
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
Nitric oxide (NO) is an important messaging molecule which plays a vital role in many biological systems. Because the endogenous NO is synthesized mainly in the mitochondria of mammalian cells, the detection of NO in mitochondria is of great importance for studying the biological functions of NO. Here, a novel fluorescence turn-on probe (MTNO) for monitoring the mitochondrial NO is designed and synthesized. The fluorescence in-tensity of MTNO exhibits a similar to 430-fold enhancement in response to NO with a detection limit as low as 14 nM. MTNO also shows good mitochondrial localization, pH insensitivity and high selectivity to NO. With the aid of MTNO, the imaging of exogenous and endogenous NO in living cells and tissue slices is achieved through two-photon microscopy.
引用
收藏
页数:9
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