Genetic dissection of major QTL for grain number per spike on chromosomes 5A and 6A in bread wheat (Triticum aestivum L.)

被引：0

作者：

Jiang, Cheng ^{[1
,2
,3
]}

Xu, Zhibin ^{[1
]}

Fan, Xiaoli ^{[1
]}

Zhou, Qiang ^{[1
]}

Ji, Guangsi ^{[1
,3
]}

Liao, Simin ^{[1
,3
]}

Wang, Yanlin ^{[1
,3
]}

Ma, Fang ^{[1
,3
]}

Zhao, Yun ^{[2
]}

Wang, Tao ^{[1
,4
]}

Feng, Bo ^{[1
]}

机构：

[1] Chinese Acad Sci, Chengdu Inst Biol, Chengdu, Peoples R China

[2] Sichuan Univ, Coll Life Sci, Chengdu, Peoples R China

[3] Univ Chinese Acad Sci, Beijing, Peoples R China

[4] Chinese Acad Sci, Innovat Acad Seed Design, Beijing, Peoples R China

来源：

FRONTIERS IN PLANT SCIENCE | 2024年 / 14卷

基金：

中国科学院西部之光基金;

关键词：

QTL mapping; BSE-Seq; grain number per spike; haplotype analysis; wheat; QUANTITATIVE TRAIT LOCI; YIELD COMPONENTS; SYNTHETIC WHEAT; FLORAL ORGAN; CROSS; TEOSINTE-BRANCHED1; TRANSCRIPTION; ARCHITECTURE; BARLEY; AUXIN;

D O I：

10.3389/fpls.2023.1305547

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Grain number per spike (GNS) is a crucial component of grain yield and plays a significant role in improving wheat yield. To identify quantitative trait loci (QTL) associated with GNS, a recombinant inbred line (RIL) population derived from the cross of Zhongkemai 13F10 and Chuanmai 42 was employed to conduct QTL mapping across eight environments. Based on the bulked segregant exome sequencing (BSE-Seq), genomic regions associated with GNS were detected on chromosomes 5A and 6A. According to the constructed genetic maps, two major QTL QGns.cib-5A (LOD = 4.35-8.16, PVE = 8.46-14.43%) and QGns.cib-6A (LOD = 3.82-30.80, PVE = 5.44-12.38%) were detected in five and four environments, respectively. QGns.cib-6A is a QTL cluster for other seven yield-related traits. QGns.cib-5A and QGns.cib-6A were further validated using linked Kompetitive Allele Specific PCR (KASP) markers in different genetic backgrounds. QGns.cib-5A exhibited pleiotropic effects on productive tiller number (PTN), spike length (SL), fertile spikelet number per spike (FSN), and ratio of grain length to grain width (GL/GW) but did not significantly affect thousand grain weight (TGW). Haplotype analysis revealed that QGns.cib-5A and QGns.cib-6A were the targets of artificial selection during wheat improvement. Candidate genes for QGns.cib-5A and QGns.cib-6A were predicted by analyzing gene annotation, spatiotemporal expression patterns, and orthologous and sequence differences. These findings will be valuable for fine mapping and map-based cloning of genes underlying QGns.cib-5A and QGns.cib-6A.

引用

页数：14

共 68 条

[1] QTL Mapping of Yield and Yield Components under Normal and Salt-stress Conditions in Bread Wheat (Triticum aestivum L.) [J].

Azadi, Amin ;

Mardi, Mohsen ;

Hervan, Eslam Majidi ;

Mohammadi, Seyed Abolghasem ;

Moradi, Foad ;

Tabatabaee, Mohammad Taghi ;

Pirseyedi, Seyed Mostafa ;

Ebrahimi, Mohsen ;

Fayaz, Farzad ;

Kazemi, Mehrbano ;

Ashkani, Sadegh ;

Nakhoda, Babak ;

Mohammadi-Nejad, Ghasem .

PLANT MOLECULAR BIOLOGY REPORTER, 2015, 33 (01) :102-120

[2] High expression of the MADS-box gene VRT2 increases the number of rudimentary basal spikelets in wheat [J].

Backhaus, Anna E. ;

Lister, Ashleigh ;

Tomkins, Melissa ;

Adamski, Nikolai M. ;

Simmonds, James ;

Macaulay, Iain ;

Morris, Richard J. ;

Haerty, Wilfried ;

Uauy, Cristobal .

PLANT PHYSIOLOGY, 2022, 189 (03) :1536-1552

[3] Relationships between grain protein content and grain yield components through quantitative trait locus analyses in a recombinant inbred line population derived from two elite durum wheat cultivars [J].

Blanco, A. ;

Mangini, G. ;

Giancaspro, A. ;

Giove, S. ;

Colasuonno, P. ;

Simeone, R. ;

Signorile, A. ;

De Vita, P. ;

Mastrangelo, A. M. ;

Cattivelli, L. ;

Gadaleta, A. .

MOLECULAR BREEDING, 2012, 30 (01) :79-92

[4]

Boden SA, 2015, NAT PLANTS, V1, P1, DOI [10.1038/NPLANTS.2014.16, 10.1038/nplants.2014.16]

[5] Mapping of quantitative trait loci determining agronomic important characters in hexaploid wheat (Triticum aestivum L.) [J].

Börner, A ;

Schumann, E ;

Fürste, A ;

Cöster, H ;

Leithold, B ;

Röder, MS ;

Weber, WE .

THEORETICAL AND APPLIED GENETICS, 2002, 105 (6-7) :921-936

[6] Wheat TILLING Mutants Show That the Vernalization Gene VRN1 Down-Regulates the Flowering Repressor VRN2 in Leaves but Is Not Essential for Flowering [J].

Chen, Andrew ;

Dubcovsky, Jorge .

PLOS GENETICS, 2012, 8 (12)

[7] TBtools: An Integrative Toolkit Developed for Interactive Analyses of Big Biological Data [J].

Chen, Chengjie ;

Chen, Hao ;

Zhang, Yi ;

Thomas, Hannah R. ;

Frank, Margaret H. ;

He, Yehua ;

Xia, Rui .

MOLECULAR PLANT, 2020, 13 (08) :1194-1202

[8] The Genome Sequence Archive Family: Toward Explosive Data Growth and Diverse Data Types [J].

Chen, Tingting ;

Chen, Xu ;

Zhang, Sisi ;

Zhu, Junwei ;

Tang, Bixia ;

Wang, Anke ;

Dong, Lili ;

Zhang, Zhewen ;

Yu, Caixia ;

Sun, Yanling ;

Chi, Lianjiang ;

Chen, Huanxin ;

Zhai, Shuang ;

Sun, Yubin ;

Lan, Li ;

Zhang, Xin ;

Xiao, Jingfa ;

Bao, Yiming ;

Wang, Yanqing ;

Zhang, Zhang ;

Zhao, Wenming .

GENOMICS PROTEOMICS & BIOINFORMATICS, 2021, 19 (04) :578-583

[9] Molecular mapping of quantitative trait loci for yield and yield components in spring wheat (Triticum aestivum L.) [J].

Cuthbert, Janice L. ;

Somers, Daryl J. ;

Brule-Babel, Anita L. ;

Brown, P. Douglas ;

Crow, Gary H. .

THEORETICAL AND APPLIED GENETICS, 2008, 117 (04) :595-608

[10] APETALA 2-like genes AP2L2 and Q specify lemma identity and axillary floral meristem development in wheat [J].

Debernardi, Juan Manuel ;

Greenwood, Julian R. ;

Finnegan, E. Jean ;

Jernstedt, Judy ;

Dubcovsky, Jorge .

PLANT JOURNAL, 2020, 101 (01) :171-187

← 1 2 3 4 5 6 7 →