3D biomimetic environment enabling ex utero trophoblast invasion and co-culture of embryos and somatic cells

被引：3

作者：

Govindasamy, Niraimathi ^{[1
]}

Long, Hongyan ^{[2
]}

Ranga, Adrian ^{[3
]}

Trappmann, Britta ^{[2
]}

Bedzhov, Ivan ^{[1
]}

机构：

[1] Max Planck Inst Mol Biomed, Embryon Self Org Res Grp, Rontgenstr 20, D-48149 Munster, Germany

[2] Max Planck Inst Mol Biomed, Bioact Mat Lab, Rontgenstr 20, D-48149 Munster, Germany

[3] Katholieke Univ Leuven, Dept Mech Engn, Lab Bioengn & Morphogenesis, Leuven, Belgium

来源：

STAR PROTOCOLS | 2023年 / 4卷 / 03期

基金：

欧洲研究理事会;

关键词：

IN-VITRO CULTURE; MOUSE BLASTOCYSTS; STEM-CELL; DERIVATION;

D O I：

10.1016/j.xpro.2023.102456

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The first direct contact between the embryo and the mother is established during implantation. This process is inaccessible for direct studies as the implanting em-bryo is concealed by the maternal tissues. Here, we present a protocol for estab-lishing a 3D biomimetic environment based on synthetic hydrogels which harbor key biomechanical properties of the uterine stroma. We describe steps for isolating and culturing embryos in PEG/DexMA hydrogel. We then detail the co-culture of embryos and endothelial cells in a microfluidic device. For complete details on the use and execution of this protocol, please refer to Govindasamy et al. (2021)1 and Ozguldez et al. (2023).2

引用

页数：20

共 21 条

[1]

Anselin L., 1998, Spatial Econometrics: Methods and Models

[2] In vitro culture of mouse blastocysts beyond the implantation stages [J].

Bedzhov, Ivan ;

Leung, Chuen Yan ;

Bialecka, Monika ;

Zernicka-Goetz, Magdalena .

NATURE PROTOCOLS, 2014, 9 (12) :2732-2739

[3]

Behringer R., 2014, MANIPULATING MOUSE E

[4] Endometrial secretion analysis identifies a cytokine profile predictive of pregnancy in IVF [J].

Boomsma, C. M. ;

Kavelaars, A. ;

Eijkemans, M. J. C. ;

Lentjes, E. G. ;

Fauser, B. C. J. M. ;

Heijnen, C. J. ;

Macklon, N. S. .

HUMAN REPRODUCTION, 2009, 24 (06) :1427-1435

[5]

Croy BA., 2014, GUIDE INVESTIGATION

[6] Derivation and characterization of mouse embryonic stem cells from permissive and nonpermissive strains [J].

Czechanski, Anne ;

Byers, Candice ;

Greenstein, Ian ;

Schrode, Nadine ;

Donahue, Leah Rae ;

Hadjantonakis, Anna-Katerina ;

Reinholdt, Laura G. .

NATURE PROTOCOLS, 2014, 9 (03) :559-574

[7] Synthesis and characterization of well-defined hydrogel matrices and their application to intestinal stem cell and organoid culture [J].

Gjorevski, Nikolce ;

Lutolf, Matthias P. .

NATURE PROTOCOLS, 2017, 12 (11) :2263-2274

[8] 3D biomimetic platform reveals the first interactions of the embryo and the maternal blood vessels [J].

Govindasamy, Niraimathi ;

Long, Hongyan ;

Jeong, Hyun-Woo ;

Raman, Ratish ;

Ozcifci, Burak ;

Probst, Simone ;

Arnold, Sebastian J. ;

Riehemann, Kristina ;

Ranga, Adrian ;

Adams, Ralf H. ;

Trappmann, Britta ;

Bedzhov, Ivan .

DEVELOPMENTAL CELL, 2021, 56 (23) :3276-+

[9]

Govindasamy N, 2019, METHODS MOL BIOL, V2006, P373, DOI 10.1007/978-1-4939-9566-0_25

[10] Test-tube embryos - mouse and human development in vitro to blastocyst stage and beyond [J].

Govindasamy, Niraimathi ;

Duethorn, Binyamin ;

Oezgueldez, Hatice O. ;

Kim, Yung S. ;

Bedzhov, Ivan .

INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY, 2019, 63 (3-5) :203-215

← 1 2 3 →