Antifungal activity of cisatracurium against fluconazole-resistant Candida isolates and its antibiofilm effects

被引：0

作者：

do Nascimento, Francisca B. A. ^{[1
,7
]}

Sa, Livia G. A. Valente ^{[1
,2
,7
]}

Neto, Joao B. de Andrade ^{[1
,2
,7
]}

Cabral, Vitoria P. F. ^{[1
,7
]}

Rodrigues, Daniel S. ^{[1
,7
]}

Barbosa, Amanda D. ^{[1
,7
]}

Moreira, Lara E. A. ^{[1
,7
]}

Oliveira, Leilson C. ^{[1
,7
]}

Silva, Anderson ^{[3
]}

Lima, Iri S. P. ^{[4
]}

Silva, Jacilene ^{[5
]}

Marinho, Emmanuel S. ^{[5
]}

Santos, Helcio S. ^{[6
]}

Cavalcanti, Bruno C. ^{[7
,8
]}

Morais, Manoel O. ^{[7
,8
]}

Helio, V. N. ^{[1
,7
]}

Silva, Cecilia R. ^{[1
,7
]}

机构：

[1] Univ Fed Ceara, Sch Pharm, BR-60430372 Fortaleza, Brazil

[2] Christus Univ Ctr, Fortaleza, CE, Brazil

[3] Higher Council Sci Res, Inst Adv Chem, Madrid, Spain

[4] Univ Fed Ceara, Fac Med, BR-63048080 Barbalha, CE, Brazil

[5] Univ Estadual Ceara, Theoret Chem & Electrochem Grp, BR-62930000 Limoeiro Do Norte, CE, Brazil

[6] State Univ Vale Acarau, Sci & Technol Ctr, Course Chem, Sobral, CE, Brazil

[7] Univ Fed Ceara, Drug Res & Dev Ctr, BR-60430275 Fortaleza, CE, Brazil

[8] Univ Fed Ceara, Dept Physiol & Pharmacol, BR-60430275 Fortaleza, CE, Brazil

来源：

FUTURE MICROBIOLOGY | 2023年

关键词：

antifungal activity; apoptosis; biofilm; Candida spp; cisatracurium; flow cytometry; mechanism of action; molecular docking; CANCER CELLS; ALBICANS; COMBINATION; INHIBITION; MECHANISMS; EVOLUTION; APOPTOSIS; DOCKING; BINDING; UPDATE;

D O I：

10.2217/fmb-2022-0224

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Aim: To evaluate the antifungal activity of cisatracurium against Candida spp. resistant to fluconazole strains in planktonic and biofilm forms, in addition to determining its mechanism of action. Materials & methods: Antifungal activity and pharmacological interactions were determined using broth microdilution methods and the mechanism of action was evaluated by flow cytometry and molecular docking. Results: Cisatracurium presented antifungal activity against Candida spp. planktonic cells due to alterations of mitochondrial transmembrane potential leading to cellular apoptosis in addition to interacting with important targets related to cellular respiration, membrane and cell wall evidenced by molecular docking. Furthermore, the drug both prevented biofilm formation and impaired mature biofilms. Conclusion: Cisatracurium exhibits potential antifungal activity against Candida spp. Tweetable abstractCisatracurium has antifungal activity against Candida spp. Its activity occurs by mitochondrial depolarization, affecting cell respiration, membrane and cell wall, leading to apoptosis.

引用

页码：649 / 660

页数：12

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