The pectinolytic activity of Burkholderia cepacia and its application in the bioscouring of cotton knit fabric

被引:5
作者
Shoily, Sabrina Samad [1 ]
Fatema, Kaniz [1 ]
Dina, Rasheda Begum [2 ,3 ]
Biswas, Anik [1 ]
Haque, Papia [3 ]
Rahman, Mohammed Mizanur [3 ,4 ]
Uddin, Md. Zulhash [2 ]
Sajib, Abu Ashfaqur [1 ]
机构
[1] Univ Dhaka, Dept Genet Engn & Biotechnol, Dhaka, Bangladesh
[2] Bangladesh Univ Text, Dept Wet Proc Engn, Dhaka, Bangladesh
[3] Univ Dhaka, Dept Appl Chem & Chem Engn, Dhaka, Bangladesh
[4] Dhaka Univ, Inst Leather Engn & Technol, Dhaka, Bangladesh
关键词
Burkholderia cepacia; Pectinase; Pectinolytic enzymes; Bioscouring; Pectin; PURIFICATION; DEGRADATION; PECTINASE; ALIGNMENT;
D O I
10.1186/s43141-023-00596-5
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Background Enzymatic catalysis in different industrial applications is often preferred over chemical methods due to various advantages, such as higher specificity, greater efficiency, and less environmental footprint. Pectinases are a group of enzymes that catalyze the degradation of pectic compounds, the key components of plant middle lamella and the primary cell wall. Pectinases have found applications in multiple industrial processes, including cotton bioscouring, fruit juice extraction and its clarification, plant fiber degumming, paper making, plant biomass liquefaction, and saccharification, among others. The purpose of this study was to taxonomically characterize a bacterial species exhibiting pectinolytic activities and assess its pectinolytic activity qualitatively and quantitatively, as well as test its bioscouring potential.Results Here, we report that Burkholderia cepacia, a previously unknown species with pectinolytic activity, exerts such activity comparable to commercially used pectinase enzymes in the textile industry, but requires less temperature for activity.Conclusion Quantitative evaluation of enzyme activity indicates the potential of the bacterial species for use in the bioscouring of cotton knit fabric.
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页数:9
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