Functionalization of Polydimethylsiloxane with Diazirine-Based Linkers for Covalent Protein Immobilization

被引:3
作者
Li, Jie [1 ]
Bi, Liting [2 ]
Musolino, Stefania F. [2 ]
Wulff, Jeremy E. [2 ]
Sask, Kyla N. [1 ,3 ]
机构
[1] McMaster Univ, Sch Biomed Engn, Hamilton, ON L8S 4L2, Canada
[2] Univ Victoria, Dept Chem, Victoria, BC V8W 3V6, Canada
[3] McMaster Univ, Dept Mat Sci & Engn, Hamilton, ON L8S 4L8, Canada
基金
加拿大自然科学与工程研究理事会;
关键词
polydimethylsiloxane; diazirine; functionalization; protein immobilization; surfacemodification; surface characterization; ANTITHROMBIN-HEPARIN COMPLEX; SURFACE MODIFICATION; POLYDOPAMINE; POLY(DIMETHYLSILOXANE); COATINGS; PDMS; ADSORPTION; NETWORKS; ACID;
D O I
10.1021/acsami.3c08013
中图分类号
TB3 [工程材料学];
学科分类号
0805 ; 080502 ;
摘要
Biomolecule attachment to solid supports is critical for biomedical devices, such as biosensors and implants. Polydimethylsiloxane (PDMS) is commonly used for these applications due to its advantageous properties. To enhance the biomolecule immobilization on PDMS, a novel technique is demonstrated using newly synthesized diazirine molecules for the surface modification of PDMS. This nondestructive process involves a reaction between diazirine molecules and PDMS through C-H insertion with thermal or ultraviolet activation. The success of the PDMS modification is confirmed by various surface characterization techniques. Bovine serum albumin (BSA) and immunoglobulin G (IgG) are strongly attached to the modified PDMS surfaces, and the amount of protein is quantified using iodine-125 radiolabeling. The results demonstrate that PDMS is rapidly functionalized, and the stability of the immobilized proteins is significantly improved with multiple types of diazirine molecules and activation methods. Confocal microscopy provides three-dimensional images of the distribution of immobilized IgG on the surfaces and the penetration of diazirine-based linkers through the PDMS substrate during the coating process. Overall, this study presents a promising new approach for functionalizing PDMS surfaces to enhance biomolecule immobilization, and its potential applications can extend to multimaterial modifications for various diagnostic and medical applications such as microfluidic devices and immunoassays with relevant bioactive proteins.
引用
收藏
页码:1 / 16
页数:16
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