Antiviral Activities of Streptomyces KSF 103 Methanolic Extracts against Dengue Virus Type-2

被引:1
作者
Zulkifli, Nurfatihah [1 ,2 ]
Khairat, Jasmine-Elanie [1 ]
Azman, Adzzie-Shazleen [3 ]
Baharudin, Nur-Faralyza Mohd [1 ]
Malek, Nurul-Adila [1 ]
Abidin, Syafiq-Asnawi Zainal [4 ]
Abubakar, Sazaly [2 ]
Hassandarvish, Pouya [2 ]
机构
[1] Univ Malaya, Fac Sci, Inst Biol Sci, Kuala Lumpur 50603, Wilayah Perseku, Malaysia
[2] Univ Malaya, Trop Infect Dis Res & Educ Ctr TIDREC, Level 2,High Impact Res HIR Bldg, Kuala Lumpur 50603, Wilayah Perseku, Malaysia
[3] Monash Univ Malaysia, Sch Sci, Jalan Lagoon Selatan, Bandar Sunway 47500, Selangor, Malaysia
[4] Monash Univ Malaysia, Jeffrey Cheah Sch Med & Hlth Sci, Jalan Lagoon Selatan, Bandar Sunway 47500, Selangor, Malaysia
来源
VIRUSES-BASEL | 2023年 / 15卷 / 08期
关键词
antivirals; arbovirus; dengue virus; Streptomyces; VITAMIN-D; IN-VITRO; PURINE; HYPOXANTHINE; REPLICATION; INFECTION; VIREMIA; AGENTS;
D O I
10.3390/v15081773
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Dengue has long been a serious health burden to the global community, especially for those living in the tropics. Despite the availability of vaccines, effective treatment for the infection is still needed and currently remains absent. In the present study, the antiviral properties of the Streptomyces sp. KSF 103 methanolic extract (Streptomyces KSF 103 ME), which consists of a number of potential antiviral compounds, were investigated against dengue virus serotype 2 (DENV-2). The effects of this extract against DENV-2 replication were determined using the quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). Findings from the study suggested that the Streptomyces KSF 103 ME showed maximum inhibitory properties toward the virus during the virus entry stage at concentrations of more than 12.5 mu g/mL. Minimal antiviral activities were observed at other virus replication stages; adsorption (42% reduction at 50 rho g/mL), post-adsorption (67.6% reduction at 50 mu g/mL), prophylactic treatment (68.4% and 87.7% reductions at 50 mu g/mL and 25 mu g/mL, respectively), and direct virucidal assay (48% and 56.8% reductions at 50 rho g/mL and 25 mu g/mL, respectively). The Streptomyces KSF 103 ME inhibited dengue virus replication with a 50% inhibitory concentration (IC50) value of 20.3 mu g/mL and an International System of Units (SI) value of 38.9. The Streptomyces KSF 103 ME showed potent antiviral properties against dengue virus (DENV) during the entry stage. Further studies will be needed to deduce the antiviral mechanisms of the Streptomyces KSF 103 ME against DENV.
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页数:15
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