Handheld ISFET Lab-on-Chip Detection of TMPRSS2-ERG and AR mRNA for Prostate Cancer Prognostics

被引：0

作者：

Broomfield, Joseph ^{[1
,2
]}

Kalofonou, Melpomeni ^{[1
]}

Franklin, Sylvia ^{[1
]}

Powell, Sue M. ^{[2
]}

Pataillot-Meakin, Thomas ^{[2
,3
,4
]}

Moser, Nicolas ^{[1
]}

Bevan, Charlotte L. ^{[2
]}

Georgiou, Pantelis ^{[1
]}

机构：

[1] Imperial Coll London, Ctr BioInspired Technol Elect & Elect Engn, London SW7 2AZ, England

[2] Imperial Coll London, Imperial Ctr Translat & Expt Med, Dept Surg & Canc, London W12 0NN, England

[3] Imperial Coll London, Dept Chem, Mol Sci Res Hub, London W12 0NN, England

[4] Imperial Coll London, Dept Bioengn, Sir Michael Uren Hub, London W12 0BZ, England

来源：

IEEE SENSORS LETTERS | 2023年 / 7卷 / 08期

关键词：

Chemical and biological sensors; biosensors; ion-sensitive field-effect transistors (ISFETs); lab-on-chip; prostate cancer; RNA; reverse transcriptase loop-mediated isothermal amplification (RT-LAMP); ANDROGEN RECEPTOR; ACTIVE SURVEILLANCE; CELL-PROLIFERATION; GENE FUSION; DNA; AMPLIFICATION;

D O I：

10.1109/LSENS.2023.3296395

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

Ion-sensitive field-effect transistors (ISFETs), in combination with unmodified complementary metal oxide semiconductors, present a point-of-care platform for clinical diagnostics and prognostics. This work illustrates the sensitive and specific detection of two circulating mRNA markers for prostate cancer: the androgen receptor and the TMPRSS2-ERG fusion using a target-specific loop-mediated isothermal amplification method. TMPRSS2-ERG and androgen receptor RNA were detected down to 3 x10(1) and 5 x10(1) copies, respectively, in under 30 min. Administration of these assays onto the ISFET lab-on-chip device was successful and the specificity of each marker was corroborated with mRNA extracted from prostate cancer cell lines.

引用

页数：4

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