Handheld ISFET Lab-on-Chip Detection of TMPRSS2-ERG and AR mRNA for Prostate Cancer Prognostics

被引：0

作者：

Broomfield, Joseph ^{[1
,2
]}

Kalofonou, Melpomeni ^{[1
]}

Franklin, Sylvia ^{[1
]}

Powell, Sue M. ^{[2
]}

Pataillot-Meakin, Thomas ^{[2
,3
,4
]}

Moser, Nicolas ^{[1
]}

Bevan, Charlotte L. ^{[2
]}

Georgiou, Pantelis ^{[1
]}

机构：

[1] Imperial Coll London, Ctr BioInspired Technol Elect & Elect Engn, London SW7 2AZ, England

[2] Imperial Coll London, Imperial Ctr Translat & Expt Med, Dept Surg & Canc, London W12 0NN, England

[3] Imperial Coll London, Dept Chem, Mol Sci Res Hub, London W12 0NN, England

[4] Imperial Coll London, Dept Bioengn, Sir Michael Uren Hub, London W12 0BZ, England

来源：

IEEE SENSORS LETTERS | 2023年 / 7卷 / 08期

关键词：

Chemical and biological sensors; biosensors; ion-sensitive field-effect transistors (ISFETs); lab-on-chip; prostate cancer; RNA; reverse transcriptase loop-mediated isothermal amplification (RT-LAMP); ANDROGEN RECEPTOR; ACTIVE SURVEILLANCE; CELL-PROLIFERATION; GENE FUSION; DNA; AMPLIFICATION;

D O I：

10.1109/LSENS.2023.3296395

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

Ion-sensitive field-effect transistors (ISFETs), in combination with unmodified complementary metal oxide semiconductors, present a point-of-care platform for clinical diagnostics and prognostics. This work illustrates the sensitive and specific detection of two circulating mRNA markers for prostate cancer: the androgen receptor and the TMPRSS2-ERG fusion using a target-specific loop-mediated isothermal amplification method. TMPRSS2-ERG and androgen receptor RNA were detected down to 3 x10(1) and 5 x10(1) copies, respectively, in under 30 min. Administration of these assays onto the ISFET lab-on-chip device was successful and the specificity of each marker was corroborated with mRNA extracted from prostate cancer cell lines.

引用

页数：4

共 28 条

[1]

Alexandrou G., 2020, IEEE INT SYMP CIRC S, P1, DOI DOI 10.1109/iscas45731.2020.9180622

[2] DU-145 and PC-3 human prostate cancer cell lines express androgen receptor: Implications for the androgen receptor functions and regulation [J].

Alimirah, F ;

Chen, JM ;

Basrawala, Z ;

Xin, H ;

Choubey, D .

FEBS LETTERS, 2006, 580 (09) :2294-2300

[3] Detection of YAP1 and AR-V7 mRNA for Prostate Cancer Prognosis Using an ISFET Lab-On-Chip Platform [J].

Broomfield, Joseph ;

Kalofonou, Melpomeni ;

Pataillot-Meakin, Thomas ;

Powell, Sue M. ;

Fernandes, Rayzel C. ;

Moser, Nicolas ;

Bevan, Charlotte L. ;

Georgiou, Pantelis .

ACS SENSORS, 2022, :3389-3398

[4] Active Surveillance for Low-Risk Prostate Cancer Worldwide: The PRIAS Study [J].

Bul, Meelan ;

Zhu, Xiaoye ;

Valdagni, Riccardo ;

Pickles, Tom ;

Kakehi, Yoshiyuki ;

Rannikko, Antti ;

Bjartell, Anders ;

van der Schoot, Deric K. ;

Cornel, Erik B. ;

Conti, Giario N. ;

Boeve, Egbert R. ;

Staerman, Frederic ;

Vis-Maters, Jenneke J. ;

Vergunst, Henk ;

Jaspars, Joris J. ;

Stroelin, Petra ;

van Muilekom, Erik ;

Schroder, Fritz H. ;

Bangma, Chris H. ;

Roobol, Monique J. .

EUROPEAN UROLOGY, 2013, 63 (04) :597-603

[5] Androgen-Induced Cell Migration: Role of Androgen Receptor/Filamin A Association [J].

Castoria, Gabriella ;

D'Amato, Loredana ;

Ciociola, Alessandra ;

Giovannelli, Pia ;

Giraldi, Tiziana ;

Sepe, Leandra ;

Paolella, Giovanni ;

Barone, Maria Vittoria ;

Migliaccio, Antimo ;

Auricchio, Ferdinando .

PLOS ONE, 2011, 6 (02)

[6] Role of Circulating Tumor Cells (CTC), Androgen Receptor Full Length (AR-FL) and Androgen Receptor Splice Variant 7 (AR-V7) in a Prospective Cohort of Castration-Resistant Metastatic Prostate Cancer Patients [J].

Cattrini, Carlo ;

Rubagotti, Alessandra ;

Zinoli, Linda ;

Cerbone, Luigi ;

Zanardi, Elisa ;

Capaia, Matteo ;

Barboro, Paola ;

Boccardo, Francesco .

CANCERS, 2019, 11 (09)

[7] Role of the TMPRSS2-ERG gene fusion in prostate cancer [J].

Chinnaiyan, Arul M. .

NEOPLASIA, 2008, 10 (02) :177-U23

[8] The need for active surveillance for low risk prostate cancer [J].

Drost, Frank-Jan H. ;

Roobol, Monique J. .

EXPERT REVIEW OF ANTICANCER THERAPY, 2017, 17 (06) :487-489

[9] Androgen receptor amplification is associated with increased cell proliferation in prostate cancer [J].

Haapala, Kyllikki ;

Kuukasjarvi, Tuuta ;

Hyytinen, Eija ;

Rantala, Immo ;

Helin, Heikki J. ;

Koivisto, Pasi A. .

HUMAN PATHOLOGY, 2007, 38 (03) :474-478

[10] Androgen receptor and its splice variants in prostate cancer [J].

Haile, Simon ;

Sadar, Marianne D. .

CELLULAR AND MOLECULAR LIFE SCIENCES, 2011, 68 (24) :3971-3981

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