Analysis of Genome Variability of Escherichia coli When Exposed to Ionizing Radiation

被引：0
作者：
Gallyamova, M. Yu. [1 ]
Vagin, K. N. [1 ,2 ]
Vasilevsky, N. M. [1 ]
Hammadov, N. I. [1 ]
机构：
[1] Fed Ctr Toxicol Radiat & Biol Safety, Kazan 420075, Russia
[2] Kazan Volga Reg Fed Univ, Kazan 420008, Russia
来源：
RUSSIAN JOURNAL OF GENETICS | 2024年 / 60卷 / 01期
关键词：
genome; ionizing radiation; Escherichia coli PL-6; DNA damage; polymerase chain reaction; RESISTANCE;
D O I：
10.1134/S1022795424010058
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
The study of changes in the genetic apparatus of biological systems due to the influence of physical factors contributes to understanding the mechanisms of adaptation. The article is devoted to the analysis of the genome of the modified Escherichia coli PL-6 variant obtained as a result of repeated and gradually increasing exposure to Co-60 gamma rays at the Researcher facility. The integrity of the genetic material of the studied bacterial cells was checked by electrophoresis in 1.7% agarose gel. For the analysis of genome modifications, primers were designed to amplify several loci characterized by homology in a variety of E. coli strains. On the basis of the indicators of the number and size of amplified products using each of the presented primer combinations in E. coli before and after gamma irradiation, a significant change in the genome was established.
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页码：49 / 55
页数：7
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