Exosomes Derived from Adipose Stem Cells Enhance Angiogenesis in Diabetic Wound Via miR-146a-5p/JAZF1 Axis

被引：7
作者：
Che, Dehui [1 ]
Xiang, Xinjian [1 ]
Xie, Juan [1 ]
Chen, Zenghong [1 ]
Bao, Qiong [1 ]
Cao, Dongsheng [1 ]
机构：
[1] Anhui Med Univ, Affiliated Hosp 2, Dept Plast & Reconstruct Surg, Hefei 230601, Peoples R China
来源：
STEM CELL REVIEWS AND REPORTS | 2024年 / 20卷 / 04期
关键词：
Adipose stem cells; Exosomes; miR-146a-5p; JAZF1; Angiogenesis; Diabetic wound; EXTRACELLULAR VESICLES; RECEPTOR; PROLIFERATION; MIR-146A;
D O I：
10.1007/s12015-024-10685-8
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
Chronic trauma in diabetes is a leading cause of disability and mortality. Exosomes show promise in tissue regeneration. This study investigates the role of exosomes derived from adipose stem cells (ADSC-Exos) in angiogenesis. MiRNA-seq analysis revealed significant changes in 47 genes in human umbilical vein endothelial cells (HUVECs) treated with ADSC-Exos, with miR-146a-5p highly expressed. MiR-146a-5p mimics enhanced the pro-angiogenic effects of ADSC-Exos, while inhibitors had the opposite effect. JAZF1 was identified as a direct downstream target of miR-146a-5p through bioinformatics, qRT-PCR, and dual luciferase assay. Overexpress of JAZF1 resulted in decreased proliferation, migration, and angiogenic capacity of HUVECs, and reduced VEGFA expression. This study proposes that ADSC-Exos regulate angiogenesis partly via the miR-146a-5p/JAZF1 axis.
引用
收藏
页码：1026 / 1039
页数：14
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