Exosomes Derived from Adipose Stem Cells Enhance Angiogenesis in Diabetic Wound Via miR-146a-5p/JAZF1 Axis

被引：13

作者：

Che, Dehui ^{[1
]}

Xiang, Xinjian ^{[1
]}

Xie, Juan ^{[1
]}

Chen, Zenghong ^{[1
]}

Bao, Qiong ^{[1
]}

Cao, Dongsheng ^{[1
]}

机构：

[1] Anhui Med Univ, Affiliated Hosp 2, Dept Plast & Reconstruct Surg, Hefei 230601, Peoples R China

来源：

STEM CELL REVIEWS AND REPORTS | 2024年 / 20卷 / 04期

关键词：

Adipose stem cells; Exosomes; miR-146a-5p; JAZF1; Angiogenesis; Diabetic wound; EXTRACELLULAR VESICLES; RECEPTOR; PROLIFERATION; MIR-146A;

D O I：

10.1007/s12015-024-10685-8

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Chronic trauma in diabetes is a leading cause of disability and mortality. Exosomes show promise in tissue regeneration. This study investigates the role of exosomes derived from adipose stem cells (ADSC-Exos) in angiogenesis. MiRNA-seq analysis revealed significant changes in 47 genes in human umbilical vein endothelial cells (HUVECs) treated with ADSC-Exos, with miR-146a-5p highly expressed. MiR-146a-5p mimics enhanced the pro-angiogenic effects of ADSC-Exos, while inhibitors had the opposite effect. JAZF1 was identified as a direct downstream target of miR-146a-5p through bioinformatics, qRT-PCR, and dual luciferase assay. Overexpress of JAZF1 resulted in decreased proliferation, migration, and angiogenic capacity of HUVECs, and reduced VEGFA expression. This study proposes that ADSC-Exos regulate angiogenesis partly via the miR-146a-5p/JAZF1 axis.

引用

页码：1026 / 1039

页数：14

共 38 条

[1] Human bone marrow- and adipose-mesenchymal stem cells secrete exosomes enriched in distinctive miRNA and tRNA species [J].

Baglio, Serena Rubina ;

Rooijers, Koos ;

Koppers-Lalic, Danijela ;

Verweij, Frederik J. ;

Lanzon, M. Perez ;

Zini, Nicoletta ;

Naaijkens, Benno ;

Perut, Francesca ;

Niessen, Hans W. M. ;

Baldini, Nicola ;

Pegtel, D. Michiel .

STEM CELL RESEARCH & THERAPY, 2015, 6

[2] MicroRNA 146a is associated with diabetic complications in type 1 diabetic patients from the EURODIAB PCS [J].

Barutta, Federica ;

Corbetta, Beatrice ;

Bellini, Stefania ;

Guarrera, Simonetta ;

Matullo, Giuseppe ;

Scandella, Michela ;

Schalkwijk, Casper ;

Stehouwer, Coen D. ;

Chaturvedi, Nish ;

Soedamah-Muthu, Sabita S. ;

Durazzo, Marilena ;

Gruden, Gabriella .

JOURNAL OF TRANSLATIONAL MEDICINE, 2021, 19 (01)

[3] miR-146a-5p Promotes Angiogenesis and Confers Trastuzumab Resistance in HER2+Breast Cancer [J].

Cabello, Paula ;

Torres-Ruiz, Sandra ;

Adam-Artigues, Anna ;

Fores-Martos, Jaume ;

Martinez, Maria Teresa ;

Hernando, Cristina ;

Zazo, Sandra ;

Madoz-Gurpide, Juan ;

Rovira, Ana ;

Burgues, Octavio ;

Rojo, Federico ;

Albanell, Joan ;

Lluch, Ana ;

Bermejo, Begona ;

Cejalvo, Juan Miguel ;

Eroles, Pilar .

CANCERS, 2023, 15 (07)

[4] Extracellular Vesicles Derived From Mesenchymal Stem Cells (MSC) in Regenerative Medicine: Applications in Skin Wound Healing [J].

Casado-Diaz, Antonio ;

Quesada-Gomez, Jose Manuel ;

Dorado, Gabriel .

FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, 2020, 8

[5] Experimental study on the influence of different aperture connectors on nanofat [J].

Che, Dehui ;

Zhou, Yongting ;

Wang, Jie ;

Liu, Ying ;

Gao, Feng ;

Lv, Tianqi ;

Cui, Chenyang ;

Xiao, Zhibo .

JOURNAL OF PLASTIC RECONSTRUCTIVE AND AESTHETIC SURGERY, 2022, 75 (09) :3595-3602

[6] Extracellular vesicles: biology and emerging therapeutic opportunities [J].

EL Andaloussi, Samir ;

Maeger, Imre ;

Breakefield, Xandra O. ;

Wood, Matthew J. A. .

NATURE REVIEWS DRUG DISCOVERY, 2013, 12 (05) :348-358

[7] Extracellular Vesicles From Adipose Stem Cells Prevent Muscle Damage and Inflammation in a Mouse Model of Hind Limb Ischemia Role of Neuregulin-1 [J].

Figliolini, Federico ;

Ranghino, Andrea ;

Grange, Cristina ;

Cedrino, Massimo ;

Tapparo, Marta ;

Cavallari, Claudia ;

Rossi, Andrea ;

Togliatto, Gabriele ;

Femmino, Saveria ;

Gugliuzza, Maria Vittoria ;

Camussi, Giovanni ;

Brizzi, Maria Felice .

ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 2020, 40 (01) :239-254

[8] First pilot case-control interventional study using autologous extracellular vesicles to treat chronic venous ulcers unresponsive to conventional treatments [J].

Gibello, Lorenzo ;

D'Antico, Sergio ;

Salafia, Marika ;

Senetta, Rebecca ;

Pomatto, Margherita Alba Carlotta ;

Orlando, Giulia ;

Sarcinella, Alessandro ;

Lopatina, Tatiana ;

Quaglino, Pietro ;

Lorenzi, Marco ;

Verzini, Fabio ;

Camussi, Giovanni ;

Brizzi, Maria Felice .

PHARMACOLOGICAL RESEARCH, 2023, 190

[9] Orphan nuclear receptors in angiogenesis and follicular development [J].

Guzman, Adrian ;

Hughes, Camilla H. K. ;

Murphy, Bruce D. .

REPRODUCTION, 2021, 162 (03) :R35-R54

[10] The Kinetics of Small Extracellular Vesicle Delivery Impacts Skin Tissue Regeneration [J].

Henriques-Antunes, Helena ;

Cardoso, Renato M. S. ;

Zonari, Alessandra ;

Correia, Joana ;

Leal, Ermelindo C. ;

Jimenez-Balsa, Adrian ;

Lino, Miguel M. ;

Barradas, Ana ;

Kostic, Ivana ;

Gomes, Celia ;

Karp, Jeffrey M. ;

Carvalho, Eugenia ;

Ferreira, Lino .

ACS NANO, 2019, 13 (08) :8694-8707

← 1 2 3 4 →