Aerococcus agrisoli sp. nov., isolated from paddy soil

A non-spore-forming, Gram-stain-positive, short rod-shaped strain, designated SJQ22(T), was isolated from a paddy soil sample collected in Shanghai, PR China. A comparative analysis of 16S rRNA gene sequences showed that strain SJQ22T fell within the genus Aerococcus, forming a clear cluster with the type strains of Aerococcus viridans (98.6 % sequence similarity) and Aerococcus urinaeequi (98.5% sequence similarity). Strain SJQ22(T) grew at 30-45 degrees C (optimum, 30 degrees C), pH 6.0-8.0 (optimum, pH 7.0) and with a NaCl concentration of 0-4% (optimum, 1 %). Cells were negative for oxidase and catalase activity. Chemotaxonomic analysis showed that strain SJQ22(T) possessed C-16:0 and C-18:1 omega 9c as the predominant fatty acids. The DNA G + C content was 39.0 mol%. Strain SJQ22(T) exhibited DNA-DNA relatedness levels of 13 +/- 2% with A. viridans ATCC 11563(T) and 9 +/- 2% with A. urinaeequi IFO 12173(T). Based on the data obtained, strain SJQ22(T) represents a novel species of the genus Aerococcus, for which the name Aerococcus agrisoli sp. nov. is proposed. The type strain is SJQ22(T) (=JCM 33111(T)=CCTCC AB 2018283(T)).