Characterization of Growth-Promoting Activities of Consortia of Chlorpyrifos Mineralizing Endophytic Bacteria Naturally Harboring in Rice Plants-A Potential Bio-Stimulant to Develop a Safe and Sustainable Agriculture

被引：5

作者：

Prodhan, Md. Yeasin ^{[1
]}

Rahman, Md. Bokhtiar ^{[1
]}

Rahman, Aminur ^{[2
]}

Akbor, Md. Ahedul ^{[3
]}

Ghosh, Sibdas ^{[4
]}

Nahar, Mst. Nur-E-Nazmun ^{[1
]}

Simo, Md.

Shamsuzzoha, Md. ^{[5
]}

Cho, Kye Man ^{[6
,7
]}

Haque, Md. Azizul ^{[1
]}

机构：

[1] Hajee Mohammad Danesh Sci & Technol Univ, Dept Biochem & Mol Biol, Dinajpur 5200, Bangladesh

[2] King Faisal Univ, Coll Clin Pharm, Dept Biomed Sci, Al Hasa 31982, Saudi Arabia

[3] Bangladesh Council Sci & Ind Res BCSIR, Inst Natl Analyt Res & Serv INARS, Dhaka 1205, Bangladesh

[4] Carlow Univ, Coll Arts & Sci, Dept Biol Sci, 3333 Fifth Ave, Pittsburgh, PA 15213 USA

[5] Hajee Mohammad Danesh Sci & Technol Univ, Dept Chem, Dinajpur 5200, Bangladesh

[6] Gyeongsang Natl Univ, Dept Green Bio Sci, Jinju 52725, South Korea

[7] Gyeongsang Natl Univ, Agrifood Bio Convergence Inst, Jinju 52725, South Korea

来源：

MICROORGANISMS | 2023年 / 11卷 / 07期

关键词：

pesticide-degrading endophyte; growth promotion; MDR bacterial inhibition; synthetic consortia; GC-MS; MS analysis; rice plant; yields enhancement; CARBAMATE PESTICIDE-RESIDUES; SYSTEMIC RESISTANCE; MEDICINAL-PLANT; WATER SAMPLES; ORGANOPHOSPHORUS; STRAINS; BIODEGRADATION; INOCULATION; DEGRADATION; BACILLUS;

D O I：

10.3390/microorganisms11071821

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Eighteen pesticide-degrading endophytic bacteria were isolated from the roots, stems, and leaves of healthy rice plants and identified through 16S rRNA gene sequencing. Furthermore, biochemical properties, including enzyme production, dye degradation, anti-bacterial activities, plant-growth-promoting traits, including N-fixation, P-solubilization, auxin production, and ACC-deaminase activities of these naturally occurring endophytic bacteria along with their four consortia, were characterized. Enterobacter cloacae HSTU-ABk39 and Enterobacter sp. HSTU-ABk36 displayed inhibition zones of 41.5 & PLUSMN; 1.5 mm, and 29 & PLUSMN; 09 mm against multidrug-resistant human pathogenic bacteria Staphylococcus aureus and Staphylococcus epidermidis, respectively. FT-IR analysis revealed that all eighteen isolates were able to degrade chlorpyrifos pesticide. Our study confirms that pesticide-degrading endophytic bacteria from rice plants play a key role in enhancing plant growth. Notably, rice plants grown in pots containing reduced urea (30%) mixed with either endophytic bacterial consortium-1, consortium-2, consortium-3, or consortia-4 demonstrated an increase of 17.3%, 38.6%, 18.2%, and 39.1% yields, respectively, compared to the control plants grown in pots containing 100% fertilizer. GC-MS/MS analysis confirmed that consortia treatment caused the degradation of chlorpyrifos into different non-toxic metabolites, including 2-Hydroxy-3,5,6 trichloropyridine, Diethyl methane phosphonate, Phorate sulfoxide, and Carbonochloridic. Thus, these isolates could be deployed as bio-stimulants to improve crop production by creating a sustainable biological system.

引用

页数：29