Expression and in vitro function of anti-PD-L1 human antibody expressed in plant

被引：5

作者：

Lee, Chae Eun ^{[1
]}

Lee, Jeong Hwan ^{[1
]}

Chung, Hyun Joo ^{[1
]}

Lee, Da Won ^{[1
]}

Lim, Jong Seok ^{[1
]}

Kim, Kibum ^{[3
]}

Kim, Jin Wook ^{[4
,5
]}

Lee, Yong Seong ^{[4
]}

Kim, Kyung Soo ^{[6
]}

Min, Hyun Jin ^{[6
]}

Ko, Kisung ^{[2
,3
]}

Myung, Soon Chul ^{[1
,2
]}

机构：

[1] Chung Ang Univ, Coll Med, Dept Urol, Seoul, South Korea

[2] Chung Ang Univ, Mol Biodesign Res Ctr, Seoul, South Korea

[3] Chung Ang Univ, Coll Med, Dept Med, Therapeut Prot Engn Lab, Seoul, South Korea

[4] Chung Ang Univ, Dept Urol, Gwangmyeong Hosp, Gyeonggi Do, South Korea

[5] Chung Ang Univ, Coll Med, Dept Med Informat, Seoul, South Korea

[6] Chung Ang Univ, Coll Med, Dept Otorhinolaryngol Head & Neck Surg, Seoul, South Korea

来源：

PLANT BIOTECHNOLOGY REPORTS | 2023年 / 17卷 / 04期

基金：

新加坡国家研究基金会;

关键词：

Anti-PD-L1; antibodies; Avelumab; Immune system; Plant expression system; Therapeutic protein; VACCINES;

D O I：

10.1007/s11816-023-00844-7

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Plants can be used to produce recombinant functional proteins such as antibodies and vaccines. In this study, transgenic plants were utilized to express anti-human programmed death ligand-1 (PD-L1) monoclonal antibodies (mAb(P) PD-L1) tagged with the endoplasmic reticulum retention motif KDEL. PCR and immunoblot analyses confirmed that mAb PD-L1 was expressed in transgenic plants. ELISA analysis confirmed the binding activity of mAb(P) PD-L1 to human prostate and urothelial cancer cell lines PC-3 and TCCSUP, respectively. Confocal imaging analysis demonstrated that mAb(P) PD-L1 had binding activity against human cancer cell lines similar to Avelumab (mAb(M) PD-L1). Confocal imaging analysis showed that the binding activity of mAb(P) PD-L1 to the human tonsil is comparable to mAb(M) PD-L1. These results suggest that mAb(P) PD-L1 shows functional binding activities for PD-L1(+) cancer cells. Hence, this study showed that tobacco plants can be used to produce functional anti-human PD-L1 mAbs.

引用

页码：531 / 539

页数：9

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