Isocratic elution based analytical method for quantification of phytoconstituents in Hedychium spicatum and evaluation of their α-glucosidase inhibitory activity

An efficient, fast, selective and simple isocratic elution method has been established using ultra performance liquid chromatography coupled to photodiode array detection and electrospray ionisation mass spectrometry (UPLC-PDA-ESI-MS) techniques for the quantification of isolated molecules from Hedychium spicatum rhizomes and assess their ct-glucosidase inhibitory activity. Developed analytical method was validated as per the regulatory guidelines such as linearity, selectivity, the limits of detection (LOD) and quantification (LOQ), repeatability and accuracy. Highest concentration of 7-hydroxy hedychenone (4) (76.7 mg/g of dried plant material) was detected in rhizomes. Present results demonstrated parent extract (ethanolic) had the greatest inhibitory effect on alpha-glucosidase with IC50 values of 4.0 mu g/ml. Developed method can be used as a new analytical approach for quality assessment of H. spicatum based herbal formulations and other products. Furthermore, current findings showed that the extract, fraction and isolated metabolites may be a good natural antidiabetic. [GRAPHICS] .