Wide-scope targeted analysis of bioactive lipids in human plasma by LC/MS/MS

被引：8

作者：

Nakatani, Kohta ^{[1
]}

Izumi, Yoshihiro ^{[1
]}

Umakoshi, Hironobu ^{[2
]}

Yokomoto-Umakoshi, Maki ^{[2
]}

Nakaji, Tomoko

Kaneko, Hiroki ^{[2
]}

Nakao, Hiroshi ^{[2
]}

Ogawa, Yoshihiro ^{[2
]}

Ikeda, Kazutaka ^{[3
]}

Bamba, Takeshi ^{[1
]}

机构：

[1] Kyushu Univ, Med Inst Bioregulat, Mass Spectrometry Ctr, Med Res Ctr High Depth Om,Div Metab, Fukuoka, Japan

[2] Kyushu Univ, Grad Sch Med Sci, Dept Med & Bioregulatory Sci, Fukuoka, Japan

[3] Kazusa DNA Res Inst, Dept Appl Genom, Lab Biomol Anal, Kisarazu, Chiba, Japan

来源：

JOURNAL OF LIPID RESEARCH | 2024年 / 65卷 / 01期

基金：

日本科学技术振兴机构; 日本学术振兴会;

关键词：

steroid; bile acid; polyunsaturated fatty acid; liquid chromatography; tandem mass spectrometry; solid-phase extraction; comprehensive analysis; LC-MS/MS ANALYSIS; MASS-SPECTROMETRY; BEHAVIOR;

D O I：

10.1016/j.jlr.2023.100492

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Quantitative information on blood metabolites can be used in developing advanced medical strategies such as early detection and prevention of disease. Monitoring bioactive lipids such as steroids, bile acids, and PUFA metabolites could be a valuable indicator of health status. However, a method for simultaneously measuring these bioactive lipids has not yet been developed. Here, we report a LC/MS/MS method that can simultaneously measure 144 bioactive lipids, including steroids, bile acids, and PUFA metabolites, from human plasma, and a sample preparation method for these targets. Protein removal by methanol precipitation and purification of bioactive lipids by solid-phase extraction improved the recovery of the targeted compounds in human plasma samples, demonstrating the importance of sample preparation methods for a wide range of bioactive lipid analyses. Using the developed method, we studied the plasma from healthy human volunteers and confirmed the presence of bioactive lipid molecules associated with sex differences and circadian rhythms. The developed method of bioactive lipid analysis can be applied to health monitoring and disease biomarker discovery in precision medicine.

引用

页数：13

共 35 条

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