HER2 aptamer-conjugated iron oxide nanoparticles with PDMAEMA-b-PMPC coating for breast cancer cell identification

被引：3

作者：

Negrao, Cyro von Zuben de Valega ^{[1
,2
,3
]}

Cerize, Natalia N. P. ^{[3
]}

Justo Jr, Amauri da Silva ^{[2
]}

Liszbinski, Raquel Bester ^{[1
,2
]}

Meneguetti, Giovanna Pastore ^{[3
]}

Araujo, Larissa ^{[3
]}

Rocco, Silvana A. ^{[2
]}

Goncalves, Kaliandra de Almeida ^{[2
]}

Cornejo, Daniel R. ^{[4
]}

Leo, Patricia ^{[3
]}

Perecin, Caio ^{[3
]}

Adamoski, Douglas ^{[2
]}

Dias, Sandra M. Gomes ^{[2
]}

机构：

[1] Univ Campinas UNICAMP, Inst Biol, Grad Program Genet & Mol Biol, BR-13083864 Campinas, SP, Brazil

[2] Brazilian Ctr Res Energy & Mat CNPEM, Brazilian Biosci Natl Lab LNBio, BR-13083970 Campinas, SP, Brazil

[3] Inst Technol Res IPT, Bionanomanufacturing Ctr, BR-05508901 Sao Paulo, SP, Brazil

[4] Univ Sao Paulo, Inst Phys, Dept Mat & Mech, BR-05508090 Sao Paulo, SP, Brazil

来源：

NANOMEDICINE | 2024年 / 19卷 / 03期

基金：

巴西圣保罗研究基金会;

关键词：

aptamers; block copolymers; breast cancer; diagnostics; hybrid nanoparticles; RAFT-POLYMERIZATION; REVERSIBLE-ADDITION; MOLECULAR-WEIGHT; IN-VITRO; METHACRYLATE; COPOLYMERS; DELIVERY; FUNCTIONALIZATION; CYTOTOXICITY; CHALLENGES;

D O I：

10.2217/nnm-2023-0225

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Aim: To synthesize HER2 aptamer-conjugated iron oxide nanoparticles with a coating of poly(2-(dimethylamino) ethyl methacrylate)-poly(2-methacryloyloxyethylphosphorylcholine) block copolymer (IONPPPs). Methods: Characterization covered molecular structure, chemical composition, thermal stability, magnetic characteristics, aptamer interaction, crystalline nature and microscopic features. Subsequent investigations focused on IONPPPs for in vitro cancer cell identification. Results: Results demonstrated high biocompatibility of the diblock copolymer with no significant toxicity up to 150 mu g/ml. The facile coating process yielded the IONPP complex, featuring a 13.27 nm metal core and a 3.10 nm polymer coating. Functionalized with a HER2-targeting DNA aptamer, IONPPP enhanced recognition in HER2-amplified SKBR3 cells via magnetization separation. Conclusion: These findings underscore IONPPP's potential in cancer research and clinical applications, showcasing diagnostic efficacy and HER2 protein targeting in a proof-of-concept approach.

引用

页码：231 / 254

页数：24

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