Airway epithelium regeneration by photoactivated basal cells

被引：2

作者：

Wang, Shaoyang ^{[1
,2
,3
]}

Shan, Shan ^{[1
]}

Zhang, Jingyuan ^{[1
,3
]}

Liu, Zeyu ^{[1
]}

Gu, Xiaohua ^{[1
]}

Hong, Yue ^{[4
,5
]}

He, Hao ^{[3
]}

Ren, Tao ^{[1
,6
]}

机构：

[1] Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 6, Sch Med, Dept Resp Med,Dept Orthopaed, 600 Yishan Rd, Shanghai 200233, Peoples R China

[2] Hainan Univ, Sch Biomed Engn, 58 Renmin Ave, Haikou 570228, Hainan, Peoples R China

[3] Shanghai Jiao Tong Univ, Sch Biomed Engn, 1954 Huashan Rd, Shanghai 200030, Peoples R China

[4] Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 6, Stem Cell Ctr, Sch Med,Ctr Sleep Med, 600 Yishan Rd, Shanghai 200233, Peoples R China

[5] Hainan Univ, Sch Life Sci, 58 Renmin Ave, Haikou 570228, Peoples R China

[6] Shanghai Key Lab Sleep Disordered Breathing, 600 Yishan Rd, Shanghai 200233, Peoples R China

来源：

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY | 2023年 / 245卷

基金：

中国国家自然科学基金;

关键词：

Airway basal cell; Photoactivation; Airway regeneration; Femtosecond laser; STEM-CELLS; LUNG; PROLIFERATION; MECHANISMS; REPAIR; PLASTICITY;

D O I：

10.1016/j.jphotobiol.2023.112732

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The airway epithelium is the footstone to maintain the structure and functions of lung, in which resident basal cells (BCs) maintain homeostasis and functional regeneration of epithelial barrier in response to injury. In recent clinical researches, transplanting BCs has shown great inspiring achievements in therapy of various lung diseases. In this study, we report a noninvasive optical method to activate BCs for airway epithelium regeneration in vivo by fast scanning of focused femtosecond laser on BCs of airway epithelium to active Ca2+ signaling and subse-quent ERK and Wnt pathways. The photoactivated BCs present high proliferative capacity and maintain high pluripotency, which enables them to plant in the injured airway epithelium and differentiate to club cells for regeneration of epithelium. This optical method can also work in situ to activate localized BCs in airway tissue. Therefore, our results provide a powerful technology for noninvasive BC activation in stem-cell therapy of lung diseases.

引用

页数：10

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