The Impact of Acute Low-Dose Gamma Irradiation on Biomass Accumulation and Secondary Metabolites Production in Cotinus coggygria Scop. and Fragaria x ananassa Duch. Red Callus Cultures

被引：4

作者：

Ciocan, Alexandra-Gabriela ^{[1
,2
]}

Maximilian, Carmen ^{[1
]}

Mitoi, Elena Monica ^{[1
]}

Moldovan, Radu-Cristian ^{[3
]}

Negut, Daniel ^{[4
]}

Iuga, Cristina-Adela ^{[3
,5
]}

Helepciuc, Florenta Elena ^{[1
]}

Holobiuc, Irina ^{[1
]}

Radu, Mihai ^{[6
]}

Dimov, Tatiana Vassu ^{[2
]}

Cogalniceanu, Gina ^{[1
]}

机构：

[1] Romanian Acad, Inst Biol Bucharest, Dept Dev Biol, 296 Splaiul Independentei St, Bucharest 060031, Romania

[2] Univ Bucharest, Fac Biol, Splaiul Independentei 91 95, Bucharest 050095, Romania

[3] Iuliu Hatieganu Univ Med & Pharm Cluj Napoca, Res Ctr Adv Med MedFuture, Dept Prote & Metabol, 4-6 Louis Pasteur St, Cluj Napoca 400349, Romania

[4] Horia Hulubei Natl Inst R&D Phys & Nucl Engn, IRASM Radiat Proc Dept, Reactorului St 30, Magurele 077125, Romania

[5] Iuliu Hatieganu Univ Med & Pharm, Dept Pharmaceut Anal, Fac Pharm, Louis Pasteur St 6, Cluj Napoca 400349, Romania

[6] Horia Hulubei Natl Inst R&D Phys & Nucl Engn, Dept Life & Environm Phys, Reactorului St 30, RO-077125 Magurele, Romania

来源：

METABOLITES | 2023年 / 13卷 / 08期

关键词：

Cotinus coggygria; Fragaria x ananassa; callus culture; secondary metabolism; gamma irradiation; UPLC-HRMS; polyphenols; flavonoids; antioxidant activity; CHEMICAL-COMPOSITION; OXIDATIVE STRESS; ESSENTIAL OILS; PANAX-GINSENG; SMOKE TREE; STRAWBERRY; ANTIOXIDANT; LEAVES; ACID; IMPROVEMENT;

D O I：

10.3390/metabo13080894

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cotinus coggygria Scop. (smoketree) and Fragaria x ananassa Duch. (strawberry) are two industrially important species due to their composition in bioactive compounds. In this study, we investigated the effects of acute low-dose gamma irradiation (15, 20, 25, 30, 35 and 40 Gy) on two red callus cultures established in smoketree and strawberry. The biomass production, dry weight, content of phenols, flavonoids, monomeric anthocyanins', index of anthocyanins polymerization and antioxidant activity were evaluated. For the smoketree callus, a negative correlation between irradiation doses and callus biomass accumulation was observed. For the strawberry callus, irradiation did not significantly affect the accumulation of the biomass. An increased dry weight was observed in irradiated smoketree callus, while for treated strawberry callus, a decrease was recorded. Irradiation with 30 Gy was stimulative for polyphenols' accumulation in both cultures; however, the increase was significant only in the strawberry callus. The flavonoids increased in the 30 Gy strawberry variants, while it significantly decreased in smoketree callus irradiated with 35 and 40 Gy. In irradiated strawberry callus, except for the 25 Gy variant (1.65 +/- 0.4 mg C-3-GE/g DW), all treatments caused an increase in anthocyanins' accumulation. In smoketree, except for the 15 Gy variant (2.14 +/- 0.66 mg C-3-GE/g DW), the irradiation determined an increase in anthocyanins synthesis, with the highest value being seen in the 20 Gy variant (2.8 +/- 0.94 mg C-3-GE/g DW). According to UPLC-HRMS investigations, an unidentified compound increased by 99% at the 30 Gy dose in strawberry callus, while in smoketree, maslinic acid increased by 51% after irradiation with 40 Gy. The results of this study showed, for the first time, the differential response of two performant callus cultures to low-dose gamma irradiation, a biotechnological method that can be used to stimulate the synthesis of important flavonoids and triterpenes.

引用

页数：20

共 80 条

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