Development and Characterization of Folic Acid-Conjugated Amodiaquine-Loaded Nanoparticles-Efficacy in Cancer Treatment

被引:20
作者
Parvathaneni, Vineela [1 ]
Shukla, Snehal K. [1 ]
Gupta, Vivek [1 ]
机构
[1] St Johns Univ, Coll Pharm & Hlth Sci, Dept Pharmaceut Sci, Queens, NY 11439 USA
关键词
amodiaquine; folic acid; cancer; nanoparticles; targeted delivery; 3D spheroid studies; repurposing; nano-repurposing; FOLATE-RECEPTOR; TARGETED DELIVERY; DRUG-DELIVERY; IN-VITRO; DOXORUBICIN; CULTURE;
D O I
10.3390/pharmaceutics15031001
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
The objective of this study was to construct amodiaquine-loaded, folic acid-conjugated polymeric nanoparticles (FA-AQ NPs) to treat cancer that could be scaled to commercial production. In this study, folic acid (FA) was conjugated with a PLGA polymer followed by the formulation of drug-loaded NPs. The results of the conjugation efficiency confirmed the conjugation of FA with PLGA. The developed folic acid-conjugated nanoparticles demonstrated uniform particle size distributions and had visible spherical shapes under transmission electron microscopy. The cellular uptake results suggested that FA modification could enhance the cellular internalization of nanoparticulate systems in non-small cell lung cancer, cervical, and breast cancer cell types. Furthermore, cytotoxicity studies showed the superior efficacy of FA-AQ NPs in different cancer cells such as MDAMB-231 and HeLA. FA-AQ NPs had better anti-tumor abilities demonstrated via 3D spheroid cell culture studies. Therefore, FA-AQ NPs could be a promising drug delivery system for cancer therapy.
引用
收藏
页数:18
相关论文
共 59 条
[1]   Folate Functionalized PLGA Nanoparticles Loaded with Plasmid pVAX1-NH36: Mathematical Analysis of Release [J].
Alejandra Gutierrez-Valenzuela, Cindy ;
Guerrero-German, Patricia ;
Tejeda-Mansir, Armando ;
Esquivel, Reynaldo ;
Guzman-Z, Roberto ;
Lucero-Acuna, Armando .
Applied Sciences-Basel, 2016, 6 (12)
[2]   Folic Acid-Functionalized, Condensed Magnetic Nanoparticles for Targeted Delivery of Doxorubicin to Tumor Cancer Cells Overexpressing the Folate Receptor [J].
Angelopoulou, Athina ;
Kolokithas-Ntoukas, Argiris ;
Fytas, Christos ;
Avgoustakis, Konstantinos .
ACS OMEGA, 2019, 4 (26) :22214-22227
[3]  
[Anonymous], Verifying Cell-Based Assays for Use with 3D Models
[4]  
[Anonymous], 2007, What Is Cancer?-NCI
[5]  
[Anonymous], LIMITATIONS CURRENT
[6]   A detailed insight of the tumor targeting using nanocarrier drug delivery system [J].
Batool, Sibgha ;
Sohail, Saba ;
Din, Fakhar Ud ;
Alamri, Ali H. ;
Alqahtani, Ahmad S. ;
Alshahrani, Mohammad A. ;
Alshehri, Mohammed A. ;
Choi, Han Gon .
DRUG DELIVERY, 2023, 30 (01)
[7]  
Bedard PL, 2020, LANCET, V395, P1078, DOI 10.1016/S0140-6736(20)30164-1
[8]   Cancer Drug Approvals That Displaced Existing Standard-of-Care Therapies, 2016-2021 [J].
Benjamin, David J. ;
Xu, Alexander ;
Lythgoe, Mark P. ;
Prasad, Vinay .
JAMA NETWORK OPEN, 2022, 5 (03)
[9]   Three-dimensional cell culture: the missing link in drug discovery [J].
Breslin, Susan ;
O'Driscoll, Lorraine .
DRUG DISCOVERY TODAY, 2013, 18 (5-6) :240-249
[10]   LOWERING THE CHOLESTEROL CONTENT OF MA104 CELLS INHIBITS RECEPTOR-MEDIATED TRANSPORT OF FOLATE [J].
CHANG, WJ ;
ROTHBERG, KG ;
KAMEN, BA ;
ANDERSON, RGW .
JOURNAL OF CELL BIOLOGY, 1992, 118 (01) :63-69