Application and Biological Evaluation of Magnetic Cell Sorting Technology

被引：0
作者：
Hong, Tian [1 ]
Li, Jing-Wen [1 ]
Li, Ren-Ai [2 ]
Chen, Er-Ning [1 ]
Zhao, Lu-Lu [1 ]
Du, Mei-Hong [1 ]
机构：
[1] Beijing Acad Sci & Technol, Beijing Ctr Phys & Chem Anal, Inst Anal & Testing, Beijing 100094, Peoples R China
[2] Beijing City Univ, Sch Biomed, Beijing 100094, Peoples R China
来源：
PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS | 2024年 / 51卷 / 01期
关键词：
magnetic cell sorting; biological evaluation; cell sorting technology; magnetic material; lymphocyte; CIRCULATING TUMOR-CELLS; PERIPHERAL-BLOOD; MONOCLONAL-ANTIBODY; MONONUCLEAR-CELLS; MATERNAL BLOOD; FETAL CELLS; T-CELLS; SEPARATION; PURIFICATION; ENRICHMENT;
D O I：
10.16476/j.pibb.2023.0009
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Magnetic cell sorting technology is a highly specific and rapid cell sorting technology using superparamagnetic nanocomposites for cell sorting, which is widely used in immunology, stem cytology, oncology, clinical medicine and other fields. Magnetic cell sorting technology is divided into positive isolation, negative isolation/untouched cell isolation, depletion, multi-step isolation and automated cell separation systems. In this review, we firstly give a brief introduction to the classification and application of magnetic cell sorting technology, then discuss several new techniques and challenges based on magnetic cell sorting in recent years, such as improving the sorting efficiency by improving the structure of magnetic materials and magnetic field structure. The necessity of biological evaluation of magnetic cell sorting products was emphatically analyzed. Through the biological evaluation, the advantages and disadvantages of magnetic cell sorting products can be understood, and the research and development ability could be improved. Therefore, 10 biological evaluation technical parameters related to magnetic cell sorting products were proposed: yield, purity, sterility, cytotoxicity, cell morphology, viability, light scattering characteristics of cells, fluorescent antibody labeling ability of cells, cell activation and cell proliferation. The 10 biological evaluation technical parameters play an important role in promoting the standardized application of magnetic cell sorting.
引用
收藏
页码：123 / 135
页数：13
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