Acute oral antioxidant consumption does not alter brachial artery flow mediated dilation in young adults independent of exercise training status

Endothelium-dependent vasodilation can be tested using a variety of shear stress paradigms, some of which may involve the production of reactive oxygen species. The purpose of this study was to compare different methods for assessing endothelial function and their specific involvement of reactive oxygen species and influence of aerobic training status. Twenty-nine (10 F) young and healthy participants (VO(2)max: 34-74 mL<middle dot>kg(-1)<middle dot>min(-1)) consumed either an antioxidant cocktail (AOC; vitamin C, vitamin E, alpha-lipoic acid) or placebo (PLA) on each of two randomized visits. Endothelial function was measured via three different brachial artery flow-mediated dilation (FMD) tests: reactive hyperemia (RH-FMD: 5 min cuff occlusion and release), sustained shear (SS-FMD: 6 min rhythmic handgrip), and progressive sustained shear (P-SS-FMD: three intensities of 3 min of rhythmic handgrip). Baseline artery diameter decreased (all tests: 3.8 +/- 0.5 to 3.7 +/- 0.6 mm, p = 0.004), and shear rate stimulus increased (during RH-FMD test, p = 0.021; during SS-FMD test, p = 0.36; during P-SS-FMD test, p = 0.046) following antioxidant consumption. However, there was no difference in FMD following AOC consumption (RH-FMD, PLA: 8.1 +/- 2.6%, AOC: 8.2 +/- 3.5%, p = 0.92; SS-FMD, PLA: 6.9 +/- 3.9%, AOC: 7.8 +/- 5.2%, p = 0.15) or FMD per shear rate slope (P-SS-FMD: PLA: 0.0039 +/- 0.0035 mm<middle dot>s(-1), AOC: 0.0032 +/- 0.0017 mm<middle dot>s(-1), p = 0.28) and this was not influenced by training status/fitness (all p > 0.60). Allometric scaling did not alter these outcomes (all p > 0.40). Reactive oxygen species may not be integral to endothelium-dependent vasodilation tested using reactive, sustained, or progressive shear protocols in young males and females, regardless of fitness level.