A posttranscriptional pathway regulates cell wall mRNA expression in budding yeast

被引：3

作者：

Bresson, Stefan ^{[1
,2
]}

Shchepachev, Vadim ^{[1
,2
]}

Tollervey, David ^{[1
]}

机构：

[1] Univ Edinburgh, Wellcome Ctr Cell Biol, Sch Biol Sci, Edinburgh EH9 3BF, Scotland

[2] Univ Edinburgh, Inst Cell Biol, Sch Biol Sci, Edinburgh EH9 3BF, Scotland

来源：

CELL REPORTS | 2023年 / 42卷 / 03期

关键词：

SACCHAROMYCES-CEREVISIAE; CYCLIN-C; TRANSCRIPTIONAL RESPONSE; CALCOFLUOR WHITE; PROTEIN; STRESS; PHOSPHORYLATION; ECHINOCANDINS; LOCALIZATION; TRAFFICKING;

D O I：

10.1016/j.celrep.2023.112184

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The fungal cell wall provides protection and structure and is an important target for antifungal compounds. A mitogen-activated protein (MAP) kinase cascade termed the cell wall integrity (CWI) pathway regulates transcriptional responses to cell wall damage. Here, we describe a posttranscriptional pathway that plays an important complementary role. We report that the RNA-binding proteins (RBPs) Mrn1 and Nab6 specifically target the 30 UTRs of a largely overlapping set of cell wall-related mRNAs. These mRNAs are downregulated in the absence of Nab6, indicating a function in target mRNA stabilization. Nab6 acts in parallel to CWI signaling to maintain appropriate expression of cell wall genes during stress. Cells lacking both pathways are hypersensitive to antifungal compounds targeting the cell wall. Deletion of MRN1 partially alleviates growth defects associated with Delta nab6, and Mrn1 has an opposing function in mRNA destabilization. Our results un-cover a posttranscriptional pathway that mediates cellular resistance to antifungal compounds.

引用

页数：17

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