Stability of APOBEC3F in the Presence of the APOBEC3 Antagonist HIV-1 Vif Increases at the Expense of Co-Expressed APOBEC3H Haplotype I

被引:5
作者
Yousefi, Maria [1 ]
Sudarsan, Arun Kumar Annan [1 ,2 ]
Gaba, Amit [1 ]
Chelico, Linda [1 ]
机构
[1] Univ Saskatchewan, Coll Med, Dept Biochem Microbiol & Immunol, Saskatoon, SK S7N 5E5, Canada
[2] Ctr Commercializat Regenerat Med CCRM, 661 Univ Ave 1002, Toronto, ON M5G 1M1, Canada
来源
VIRUSES-BASEL | 2023年 / 15卷 / 02期
基金
加拿大健康研究院;
关键词
restriction factor; HIV-1; APOBEC3; HEPATITIS-B-VIRUS; CBF-BETA; RESTRICTION; RNA; POLYMORPHISMS; HYPERMUTATION; DIMERIZATION; DEAMINATION; INHIBITION; EVOLUTION;
D O I
10.3390/v15020463
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The seven human APOBEC3 enzymes (APOBEC3A through H, excluding E) are host restriction factors. Most of the APOBEC3 enzymes can restrict HIV-1 replication with different efficiencies. The HIV-1 Vif protein combats APOBEC3-mediated restriction by inducing ubiquitination and degradation in the proteasome. APOBEC3F and APOBEC3G can hetero-oligomerize, which increases their restriction capacity and resistance to Vif. Here we determined if APOBEC3C, APOBEC3F, or APOBEC3G could hetero-oligomerize with APOBEC3H haplotype I. APOBEC3H haplotype I has a short half-life in cells due to ubiquitination and degradation by host proteins, but is also resistant to Vif. We hypothesized that hetero-oligomerization with APOBEC3H haplotype I may result in less Vif-mediated degradation of the interacting APOBEC3 and stabilize APOBEC3H haplotype I, resulting in more efficient HIV-1 restriction. Although we found that all three APOBEC3s could interact with APOBEC3H haplotype I, only APOBEC3F affected APOBEC3H haplotype I by surprisingly accelerating its proteasomal degradation. However, this increased APOBEC3F levels in cells and virions in the absence or presence of Vif and enabled APOBEC3F-mediated restriction of HIV-1 in the presence of Vif. Altogether, the data suggest that APOBEC3 enzymes can co-regulate each other at the protein level and that they cooperate to ensure HIV-1 inactivation rather than evolution.
引用
收藏
页数:13
相关论文
共 50 条
  • [1] Role of co-expressed APOBEC3F and APOBEC3G in inducing HIV-1 drug resistance
    Mohammadzadeh, Nazanin
    Love, Robin P.
    Gibson, Richard
    Arts, Eric J.
    Poon, Art F. Y.
    Chelico, Linda
    HELIYON, 2019, 5 (04)
  • [2] APOBEC3F Determinants of HIV-1 Vif Sensitivity
    Land, Allison M.
    Shaban, Nadine M.
    Evans, Leah
    Hultquist, Judd F.
    Albin, John S.
    Harris, Reuben S.
    JOURNAL OF VIROLOGY, 2014, 88 (21) : 12923 - 12927
  • [3] HIV-1 Vif Adaptation to Human APOBEC3H Haplotypes
    Ooms, Marcel
    Brayton, Bonnie
    Letko, Michael
    Maio, Susan M.
    Pilcher, Christopher D.
    Hecht, Frederick M.
    Barbour, Jason D.
    Simon, Viviana
    CELL HOST & MICROBE, 2013, 14 (04) : 411 - 421
  • [4] The Structural Interface between HIV-1 Vif and Human APOBEC3H
    Ooms, Marcel
    Letko, Michael
    Simon, Viviana
    JOURNAL OF VIROLOGY, 2017, 91 (05)
  • [5] APOBEC3D excludes APOBEC3F from HIV-1 virions by competitive binding of RNA
    Bhattacharjee, Shreoshri
    Gaba, Amit
    Chelico, Linda
    FRONTIERS IN VIROLOGY, 2024, 4
  • [6] Structural basis of antagonism of human APOBEC3F by HIV-1 Vif
    Hu, Yingxia
    Desimmie, Belete A.
    Nguyen, Henry C.
    Ziegler, Samantha J.
    Cheng, Tat Cheung
    Chen, John
    Wang, Jia
    Wang, Hongwei
    Zhang, Kai
    Pathak, Vinay K.
    Xiong, Yong
    NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2019, 26 (12) : 1176 - +
  • [7] A Single Amino Acid in Human APOBEC3F Alters Susceptibility to HIV-1 Vif
    Albin, John S.
    LaRue, Rebecca S.
    Weaver, Jessalyn A.
    Brown, William L.
    Shindo, Keisuke
    Harjes, Elena
    Matsuo, Hiroshi
    Harris, Reuben S.
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2010, 285 (52) : 40785 - 40792
  • [8] Different interaction between HIV-1 Vif and its cellular target proteins APOBEC3G/APOBEC3F
    Nagao, Tamiko
    Yamashita, Tomoki
    Miyake, Ariko
    Uchiyama, Tsuneo
    Nomaguchi, Masako
    Adachi, Akio
    JOURNAL OF MEDICAL INVESTIGATION, 2010, 57 (1-2) : 89 - 94
  • [9] The Role of RNA in HIV-1 Vif-Mediated Degradation of APOBEC3H
    Wang, Jiayi
    Becker, Jordan T.
    Shi, Ke
    Lauer, Kate V.
    Salamango, Daniel J.
    Aihara, Hideki
    Shaban, Nadine M.
    Harris, Reuben S.
    JOURNAL OF MOLECULAR BIOLOGY, 2019, 431 (24) : 5019 - 5031
  • [10] Structural perspectives on HIV-1 Vif and APOBEC3 restriction factor interactions
    Azimi, Farshad C.
    Lee, Jeffrey E.
    PROTEIN SCIENCE, 2020, 29 (02) : 391 - 406