Protocol for generation of humanized HCC mouse model and cancer-driver mutations using CRISPR-Cas9

被引：0

作者：

Zhu, Yicheng ^{[1
]}

Tahara, Stanley M. ^{[1
]}

Tsukamoto, Hidekazu ^{[2
,3
]}

Machida, Keigo ^{[1
,3
]}

机构：

[1] Univ Southern Calif, Dept Mol Microbiol & Immunol, Los Angeles, CA 90007 USA

[2] Univ Southern Calif, Dept Pathol, Los Angeles, CA 90007 USA

[3] Southern Calif Res Ctr ALPD & Cirrhosis, Los Angeles, CA 90033 USA

来源：

STAR PROTOCOLS | 2023年 / 4卷 / 04期

关键词：

HUMAN HEPATOCYTES; PROGENITOR CELLS; LIVER; VIRUS; GENES; P450;

D O I：

10.1016/j.xpro.2023.102389

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We detail procedures for generating a humanized mouse model of hepatocellular carcinoma (HCC) recapitulating genetic mutations associated with metabolic liver diseases (MLD). We humanized liver parenchymal, non-parenchymal, and hematopoietic cells. We employed CRISPR-Cas9-based ARID1A knockout and constitutively active CTNNB1 knockin combined with an alcohol Western diet to generate cancer-driver mutations commonly found in MLD-HCC patients. This HCC model facilitates the study of tumor-promoting gene-environment interactions.For complete details on the use and execution of this protocol, please refer to Yeh et al.1

引用

页数：53

共 31 条

[11] Mice with Chimeric Livers Are an Improved Model for Human Lipoprotein Metabolism
Ellis, Ewa C. S.
Nauglers, Scott
Parini, Paolo
Mork, Lisa-Mari
Jorns, Carl
Zemack, Helen
Sandblom, Anita Lovgren
Bjorkhem, Ingemar
Ericzon, Bo-Goran
Wilson, Elizabeth M.
Strom, Stephen C.
Grompe, Markus
[J]. PLOS ONE, 2013, 8 (11):
[12] Foquet L, 2017, METHODS MOL BIOL, V1506, P117, DOI 10.1007/978-1-4939-6506-9_8
[13] CXCL12 in early mesenchymal progenitors is. required for haematopoietic stem-cell maintenance
Greenbaum, Adam
Hsu, Yen-Michael S.
Day, Ryan B.
Schuettpelz, Laura G.
Christopher, Matthew J.
Borgerding, Joshua N.
Nagasawa, Takashi
Link, Daniel C.
[J]. NATURE, 2013, 495 (7440) : 227 - 230
[14] Integrated analysis of somatic mutations and focal copy-number changes identifies key genes and pathways in hepatocellular carcinoma
Guichard, Cecile
Amaddeo, Giuliana
Imbeaud, Sandrine
Ladeiro, Yannick
Pelletier, Laura
Ben Maad, Ichrafe
Calderaro, Julien
Bioulac-Sage, Paulette
Letexier, Melanie
Degos, Francoise
Clement, Bruno
Balabaud, Charles
Chevet, Eric
Laurent, Alexis
Couchy, Gabrielle
Letouze, Eric
Calvo, Fabien
Zucman-Rossi, Jessica
[J]. NATURE GENETICS, 2012, 44 (06) : 694 - U120
[15] Mesenchymal progenitor cells in mouse foetal liver regulate differentiation and proliferation of hepatoblasts
Ito, Keiichi
Yanagida, Ayaka
Okada, Ken
Yamazaki, Yuji
Nakauchi, Hiromitsu
Kamiya, Akihide
[J]. LIVER INTERNATIONAL, 2014, 34 (09) : 1378 - 1390
[16] Methylated DNA and MeCP2 recruit histone deacetylase to repress transcription
Jones, PL
Veenstra, GJC
Wade, PA
Vermaak, D
Kass, SU
Landsberger, N
Strouboulis, J
Wolffe, AP
[J]. NATURE GENETICS, 1998, 19 (02) : 187 - 191
[17] KNOX GRACE A., 1961, AMER JOUR MED TECHNOL, V27, P287
[18] Induction and inhibition of cytochromes P450 by the St. John's wort constituent hyperforin in human hepatocyte cultures
Komoroski, BJ
Zhang, SM
Cai, HB
Hutzler, JM
Frye, R
Tracy, TS
Strom, SC
Lehmann, T
Ang, CYW
Cui, YY
Venkataramanan, R
[J]. DRUG METABOLISM AND DISPOSITION, 2004, 32 (05) : 512 - 518
[19] A MODIFIED UROKINASE PLASMINOGEN-ACTIVATOR INDUCES LIVER-REGENERATION WITHOUT BLEEDING
LIEBER, A
PEETERS, MJTFDV
GOWN, A
PERKINS, J
KAY, MA
[J]. HUMAN GENE THERAPY, 1995, 6 (08) : 1029 - 1037
[20] Fibroblast Growth Factor Signaling Controls Liver Size in Mice With Humanized Livers
Naugler, Willscott E.
Tarlow, Branden D.
Fedorov, Lev M.
Taylor, Matthew
Pelz, Carl
Li, Bin
Darnell, Jennifer
Grompe, Markus
[J]. GASTROENTEROLOGY, 2015, 149 (03) : 728 - +

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