Protocol for generation of humanized HCC mouse model and cancer-driver mutations using CRISPR-Cas9

被引：0
作者：
Zhu, Yicheng [1 ]
Tahara, Stanley M. [1 ]
Tsukamoto, Hidekazu [2 ,3 ]
Machida, Keigo [1 ,3 ]
机构：
[1] Univ Southern Calif, Dept Mol Microbiol & Immunol, Los Angeles, CA 90007 USA
[2] Univ Southern Calif, Dept Pathol, Los Angeles, CA 90007 USA
[3] Southern Calif Res Ctr ALPD & Cirrhosis, Los Angeles, CA 90033 USA
来源：
STAR PROTOCOLS | 2023年 / 4卷 / 04期
关键词：
HUMAN HEPATOCYTES; PROGENITOR CELLS; LIVER; VIRUS; GENES; P450;
D O I：
10.1016/j.xpro.2023.102389
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We detail procedures for generating a humanized mouse model of hepatocellular carcinoma (HCC) recapitulating genetic mutations associated with metabolic liver diseases (MLD). We humanized liver parenchymal, non-parenchymal, and hematopoietic cells. We employed CRISPR-Cas9-based ARID1A knockout and constitutively active CTNNB1 knockin combined with an alcohol Western diet to generate cancer-driver mutations commonly found in MLD-HCC patients. This HCC model facilitates the study of tumor-promoting gene-environment interactions.For complete details on the use and execution of this protocol, please refer to Yeh et al.1
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页数：53
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