Longitudinal tracking of T cell lymphomas in mice using flow cytometry

被引：2
|
作者：
Kuczynski, Elizabeth A. [1 ]
Carnevalli, Larissa [1 ]
Sinclair, Charles [2 ]
机构：
[1] AstraZeneca, Cambridge CB2 0AA, England
[2] Flagship Pioneering, Suite 500E,55 Cambridge Pkwy, Cambridge, MA 02142 USA
来源：
STAR PROTOCOLS | 2023年 / 4卷 / 02期
关键词：
Cancer; Cell isolation; Flow Cytometry/Mass Cytometry; Immunology;
D O I：
10.1016/j.xpro.2023.102144
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
T cell hematological cancer has a complex interplay with host immune cells, but the ability to experimentally discriminate transferred cancer cells from host cells by flow cytometry is technically challenging. Here, we present a flow cytometry protocol to evaluate cancer cell and host immune phenotypes following transplant of a T cell lymphoma bearing a congenic marker (CD45.2) into a syngeneic host (CD45.1). We describe steps for isolation of primary immune cells from mice, staining preparation with flow cytometry antibody cocktails, and analysis by flow cytometry. For complete details on the use and execution of this protocol, please refer to Kuczynski et al.1
引用
收藏
页数：14
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