Characterization of Prunus Necrotic Ringspot Virus and Cherry Virus A Infecting Myrobalan Rootstock

被引:6
作者
Ben Mansour, Karima [1 ,2 ]
Kominek, Petr [1 ]
Kominkova, Marcela [1 ]
Brozova, Jana [1 ]
机构
[1] Crop Res Inst, Ecol Diagnost & Genet Resources Agriculturally Imp, Drnovska 507, Prague 16106, Czech Republic
[2] Czech Univ Life Sci Prague, Fac Agrobiol Food & Nat Resources, Dept Plant Protect, Kamycka 129, Prague 16521, Czech Republic
来源
VIRUSES-BASEL | 2023年 / 15卷 / 08期
关键词
PNRSV; CVA; phylogenetic analysis; biological assay; symptoms; NUCLEOTIDE-SEQUENCES; SWEET CHERRY; STONE FRUITS; ORCHARDS; PHOSPHORYLATION; ALIGNMENT; PROTEIN; TREES; PLUM;
D O I
10.3390/v15081723
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Prunus necrotic ringspot virus (PNRSV) and cherry virus A (CVA) are two viruses that mainly infect plants of the genus Prunus. Full-length sequences of these two viruses, collected in the Czech Republic from Prunus cerasifera plants, were obtained via HTS sequencing. Phylogenetic analyses based on the NJ method and Splitstree tools showed that the Czech PNRSV isolate (ON088600-ON088602) is a divergent isolate from other molecular groups, sharing less than 97% pairwise nucleotide identity with members of other groups. The Czech CVA isolate (ON088603) belonged to molecular subgroup III-2, clustered with isolates from non-cherry hosts, and shared the highest pairwise nucleotide identity (99.7%) with an isolate of Australian origin.
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页数:15
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