The Effect of Natural Substances Contained in Bee Products on Prostate Cancer in In Vitro Studies

被引:1
作者
Wozniak, Przemyslaw [1 ]
Kleczka, Anna [2 ]
Jasik, Krzysztof [2 ]
Kabala-Dzik, Agata [2 ]
Dzik, Radoslaw [3 ]
Stojko, Jerzy [1 ]
机构
[1] Med Univ Siles Katowice, Fac Pharmaceut Sci Sosnowiec, Dept Toxicol & Bioanal, Ostrogorska 30, PL-41200 Sosnowiec, Poland
[2] Med Univ Siles Katowice, Fac Pharmaceut Sci Sosnowiec, Dept Pathol, Ostrogorska 30, PL-41200 Sosnowiec, Poland
[3] Silesian Tech Univ, Fac Biomed Engn, Dept Biosensors & Proc Biomed Signals, Roosevelta 40, PL-41800 Zabrze, Poland
来源
MOLECULES | 2023年 / 28卷 / 15期
关键词
prostate cancer; flavonoids; apoptosis; ACID PHENETHYL ESTER; QUERCETIN INDUCES APOPTOSIS; CELL-DEATH; SELECTIVE AUTOPHAGY; OXIDATIVE STRESS; CURCUMIN; MITOCHONDRIA; MECHANISMS; INHIBITION; EPIDEMIOLOGY;
D O I
10.3390/molecules28155719
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Prostate cancer is a common cancer in men in older age groups. The WHO forecasts an increase in the incidence of prostate cancer in the coming years. Patients may not respond to treatment, and may not tolerate the side effects of chemotherapy. Compounds of natural origin have long been used in the prevention and treatment of cancer. Flavonoids obtained from natural products, e.g., propolis, are compounds with proven antibacterial and antiviral efficacy which modulate the immune response and may be useful as adjuvants in chemotherapy. The main aim of the present study was to evaluate the cytotoxic and pro-apoptotic properties of selected flavonoids on prostate cancer cells of the LNCaP line. The compounds used in this study were CAPE, curcumin (CUR), and quercetin (QUE). Mitochondrial and lysosome metabolism was assessed by the XTT-NR-SRB triple assay as well as by the fluorescent staining techniques. Staining for reactive oxygen species was performed as well. The experiment showed that each of the tested compounds has a cytotoxic effect on the LNCaP cell line. Different types of cell death were induced by the tested compounds. Apoptosis was induced by quercetin, while autophagy-specific changes were observed after using CAPE. Compounds obtained from other bee products have antiproliferative and cytotoxic activity against LNCaP prostate cancer cells.
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页数:27
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