3D-biofabricated chondrocyte-laden decellularized extracellular matrix-contained gelatin methacrylate auxetic scaffolds under cyclic tensile stimulation for cartilage regeneration

被引：15

作者：

Chen, Yi-Wen ^{[1
,2
,3
]}

Lin, Yen-Hong ^{[2
]}

Lin, Tsung-Li ^{[1
,4
,5
]}

Lee, Kai-Xing Alvin ^{[4
]}

Yu, Min-Hua ^{[2
,6
]}

Shie, Ming-You ^{[2
,7
,8
,9
]}

机构：

[1] China Med Univ, Grad Inst Biomed Sci, Taichung 406040, Taiwan

[2] China Med Univ Hosp, X Dimens Ctr Med Res & Translat, Taichung 404332, Taiwan

[3] Asia Univ, High Performance Mat Inst x Dimens Printing, Taichung 41354, Taiwan

[4] China Med Univ Hosp, Dept Orthoped, Taichung 404332, Taiwan

[5] China Med Univ, Coll Hlth Care, Dept Sports Med, Taichung 406040, Taiwan

[6] China Med Univ, Inst Translat Med & New Drug Dev, Taichung 406040, Taiwan

[7] China Med Univ, Dept Biomed Engn, Taichung, Taiwan

[8] China Med Univ, Sch Dent, Taichung 406040, Taiwan

[9] Asia Univ, Dept Bioinformat & Med Engn, Taichung 41354, Taiwan

来源：

BIOFABRICATION | 2023年 / 15卷 / 04期

关键词：

cyclic tensile stimulation; auxetic; yes-associated protein 1; decellularized matrix; biofabrication; chondrogenic; MECHANICAL-PROPERTIES; CELL; CHONDROGENESIS;

D O I：

10.1088/1758-5090/ace5e1

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Three-dimensional (3D) hydrogel constructs can mimic features of the extracellular matrix (ECM) and have tailorable physicochemical properties to support and maintain the regeneration of articular cartilage. Various studies have shown that mechanical cues affect the cellular microenvironment and thereby influence cellular behavior. In this study, we fabricated an auxetic scaffold to investigate the effect of 3D tensile stimulation on chondrocyte behavior. Different concentrations of decellularized extracellular matrix (dECM) were mixed with fish gelatin methacrylate (FGelMa) and employed for the preparation of dECM/FGelMa auxetic bio-scaffolds using 3D biofabrication technology. We show that when human chondrocytes (HCs) were incorporated into these scaffolds, their proliferation and the expression of chondrogenesis-related markers increased with dECM content. The function of HC was influenced by cyclic tensile stimulation, as shown by increased production of the chondrogenesis-related markers, collagen II and glycosaminoglycans, with the involvement of the yes-associated protein 1 signaling pathway. The biofabricated auxetic scaffold represents an excellent platform for exploring interactions between cells and their mechanical microenvironment.

引用

页数：16