Effect of providing citrus pulp-integrated diet on fecal microbiota and serum and fecal metabolome shifts in crossbred pigs

被引:6
作者
Belloumi, Dhekra [1 ,2 ]
Calvet, Salvador [2 ]
Roca, Marta Isabel [3 ]
Ferrer, Pablo [1 ]
Jimenez-Belenguer, Ana [4 ]
Cambra-Lopez, Maria [2 ]
Garcia-Rebollar, Paloma [5 ]
Climent, Eric [6 ]
Martinez-Blanch, Juan [6 ]
Tortajada, Marta [6 ]
Chenoll, Empar [6 ]
Bermejo, Almudena [7 ]
Cerisuelo, Alba [1 ]
机构
[1] Inst Valenciano Invest Agr, Ctr Invest & Tecnol Anim, Segorbe 12400, Spain
[2] Univ Politecn Valencia, Inst Anim Sci & Technol, Valencia 46022, Spain
[3] Inst Invest Sanitaria Fe, Unidad Analit, Valencia 46026, Spain
[4] Univ Politecn Valencia, Dept Biotecnol, Valencia 46022, Spain
[5] Univ Politecn Madrid, Dept Prod Agr, ETSIAAB, Madrid 28040, Spain
[6] ADM Biopolis Sl, Paterna 46980, Spain
[7] Inst Valenciano Invest Agr, Ctr Citricultura & Prod Vegetal, Moncada 46113, Spain
关键词
PROTEIN FERMENTATION; GROWTH-PERFORMANCE; SPECTROMETRY DATA; FATTY-ACIDS; GUT; FIBER; HEALTH; RELEVANCE; KEGG;
D O I
10.1038/s41598-023-44741-z
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The study aimed to assess the impact of dehydrated citrus pulp (DCP) on growth performance, fecal characteristics, fecal bacterial composition (based on 16S rRNA analysis), and fecal and serum metabolomic profiles in crossbred pigs. 80 finishing pigs Duroc x (Landrace x Large White) were fed either a control diet (C) or a diet with 240 g/kg DCP (T) for six weeks. Including DCP in diets tended to decrease feed intake, increased (p < 0.05) the concentrations of acetic and heptanoic acids and decreased (p < 0.05) fecal butyric and branched-chain fatty acid concentrations in feces. Animals fed DCP exhibited a lower abundance of the genera Clostridium and Romboutsia, while Lachnospira significantly increased. Orthogonal partial least squares discriminant analysis plotted a clear separation of fecal and serum metabolites between groups. The main discriminant fecal metabolites were associated with bacterial protein fermentation and were downregulated in T-fed pigs. In serum, DCP supplementation upregulated metabolites related to protein and fatty acids metabolism. In conclusion, the addition of DCP as an environmentally friendly source of nutrients in pig diets, resulted in modifications of fecal bacterial composition, fermentation patterns, and overall pig metabolism, suggesting improvements in protein metabolism and gut health.
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页数:19
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