Ectobacillus ponti sp. nov., a novel bacterium isolated from Pearl River Estuary

A Gram- staining- positive, aerobic, motile, and rod- shaped strain, designated SYSU M60031(T), was isolated from a Pearl River Estuary sediment sample, Guangzhou, Guangdong, China. The isolate could grow at pH 5.0-8.0 (optimum, pH 7.0), 25-37 degrees C (optimum, 28 degrees C) and in the presence of 0-1 % (w/v) NaCl (optimum, 0 %). The predominant respiratory menaquinone of SYSU M60031(T) was MK- 7. The cellular polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, one unidentified aminophospholipid, and one unidentified aminolipid. The major fatty acids (>10 % of total) were iso-C-14:0, iso-C-15:0, anteiso-C-15:0, iso-C-16:0, and C-16:0. The genomic DNA G+C content was 51.2 %. Phylogenetic analyses based on 16S rRNA gene sequences and core genes indicated that strain SYSU M60031(T) belonged to the genus Ectobacillus and showed the highest sequence similarity to Ectobacillus funiculus NAF001(T) (96.16%), followed by Ectobacillus antri SYSU K30001(T) (95.08 %). Based on the phenotypic, genotypic, and phylogenetic data, strain SYSU M60031(T) should be considered to represent a novel species of the genus Ectobacillus, for which the name Ectobacillus ponti sp. nov. is proposed. The type strain of the proposed novel isolate is SYSU M60031(T) (=CGMCC 1.19243(T) =NBRC 115614(T)).