Lipase inhibitory activity of constituents of Physalis glutinosa and Physalis latiphysa

The new labdane-type diterpenoid, physaglutinic acid (1), was isolated from the hexane extract of Physalis glutinosa. This compound is the first succinyl labdane isolated from a Physalis species. The known constituents of this plant were identified as the labdanes 12-O-acetylphysacoztomatin (3), physacoztomatin (4) and 12-epi-nicandrodiol (6). The flavonol retusin (8) was also isolated from this species. The investigation of the acetone extract of Physalis latiphysa resulted in the isolation of the known (+)- (Z)-labda-8(17),13-diene-15,16-diol (9), labdenediol (11), the epimeric mixture of physanicantriol (12) and 14 epi-physanicantriol (13), together with the sucrose esters, nicandroses B (15) and D (16). Also, a large amount of the flavonol glycoside rutin (17) was obtained from the methanol extract. The activity of eight of the isolated compounds and three of their derivatives as lipase inhibitors was determined. The mode of binding of active compounds 4, 10 and 16 was explored using molecular docking on the binding pocket of pancreatic lipase (PDB ID 1LPB).