Elaboration of massage technique for semen collection and examination of semen characteristics in chinchilla (Chinchilla lanigera)

被引:1
作者
Babarczi, Bianka [1 ]
Drobnyak, Arpad [2 ]
Barna, Judit [2 ]
Kissne, Eva Varadi [2 ]
Szabo, Zsuzsa [2 ]
Heincinger, Monika [2 ]
Kustos, Karoly [2 ]
Szoke, Zsuzsanna [1 ]
Vegi, Barbara [2 ]
机构
[1] Hungarian Univ Agr & Life Sci, Inst Genet & Biotechnol, Dept Anim Biotechnol, Reprod Bil & Toxicol Grp, Godollo, Hungary
[2] Inst Farm Anim Gene Conservat, Inst Gene Conservat Sci & Small Anim Res, Natl Ctr Biodivers & Gene Conservat, Godollo, Hungary
关键词
ELECTROEJACULATION; SPERMATOZOA; ANESTHESIA; QUALITY; 4-DEGREES-C; STORAGE;
D O I
10.1371/journal.pone.0290441
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The practice of artificial insemination for the long-tailed chinchilla has not been fully elaborated to date, and existing data available regarding their reproduction properties is contradictory. Until now, the collection of semen for chinchillas has been most-commonly obtained using electro-ejaculation methods exclusively. The primary objective of this study was the development of a manual technique for semen collection which meets all animal welfare requirements. An additional aim was to determine the basic spermatological parameters, such as motility, concentration, type and ratio of morphological abnormalities and live/dead cell ratio, under typical northern-hemisphere conditions, in Hungary. Over a 3 month period, a special massage technique was developed for the study, and using this method, the sperm parameters of 46 males were subsequently analyzed weekly for a period of one year. Approximately 66% of chinchillas responded positively to this technique, with the success rate of semen-collection attempts showing no variation between seasons. Average sperm concentration for the whole year was 935.17 million/ml using this method. Total cell motility was the highest in winter (90.3%), and the lowest in spring (84.3%). The proportion of live, intact cells were above 80% on average for the year, while the ratios of live, morphologically abnormal and dead cells were 6% and 14%, respectively. We found that midpiece abnormalities occurred in the highest proportion (0.95%-3.38%), while the head abnormalities showed the lowest ratio (0.01%-0.15%). Standard deviation among the parameters was relatively high, with the spring season proving to be the weakest in terms of sperm quality. This study has demonstrated that, semen can be successfully collected without the use of electro-ejaculation or anesthesia. Furthermore, although spermatological parameters do exhibit some fluctuation for the different times of the year, semen collected is nonetheless suitable for the purpose of artificial insemination of chinchillas at any time.
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页数:11
相关论文
共 29 条
[1]  
Bana J., 2020, Varkonyi Reproductive technologies in animals, P193
[2]  
Barnabe V.H., 1994, Rev. Fac. Med. Vet. Zoot., V31, P29, DOI [10.11606/issn.1678-4456bjvras.1994.52080, DOI 10.11606/ISSN.1678-4456BJVRAS.1994.52080]
[3]   Reproduction in chinchilla (Chinchilla lanigera): Current status of environmental control of gonadal activity and advances in reproductive techniques [J].
Busso, J. M. ;
Ponzio, M. F. ;
de Cuneo, M. Fiol ;
Ruiz, R. D. .
THERIOGENOLOGY, 2012, 78 (01) :1-11
[4]   Year-round testicular volume and semen quality evaluations in captive Chinchilla lanigera [J].
Busso, JM ;
Ponzio, MF ;
de Cuneo, MF ;
Ruiz, RD .
ANIMAL REPRODUCTION SCIENCE, 2005, 90 (1-2) :127-134
[5]   Electroejaculation in the Chinchilla (Chinchilla lanigera):: effects of anesthesia on seminal characteristics [J].
Busso, JM ;
Ponzio, MF ;
Chiaraviglio, M ;
de Cuneo, MF ;
Ruiz, RD .
RESEARCH IN VETERINARY SCIENCE, 2005, 78 (01) :93-97
[6]   Storage of Chinchilla lanigera semen at 4°C for 24 or 72 h with two different cryoprotectants [J].
Carrascosa, RE ;
Martini, AC ;
Ponzio, MF ;
Busso, JM ;
Ponce, AA ;
Lacuara, JL .
CRYOBIOLOGY, 2001, 42 (04) :301-306
[7]   Seasonal evaluations of urinary androgen metabolites and semen quality in domestic long-tailed chinchilla (Chinchilla lanigera) under natural photoperiod [J].
Dominchin, M. F. ;
Bianconi, S. ;
Ponzio, M. F. ;
de Cuneo, M. F. Fiol ;
Ruiz, R. D. ;
Busso, J. M. .
ANIMAL REPRODUCTION SCIENCE, 2014, 145 (1-2) :99-104
[8]  
Etson CJ, 2004, CAN VET J, V45, P235
[9]  
Falk AJ, 2001, CAN VET J, V42, P116
[10]   Digital massage for semen collection, evaluation and extension in Malaysian estuarine crocodile (Crocodylus porosus) [J].
Fitri, Wan-Nor ;
Wahid, Haron ;
Rinalfi, Putra Tengku ;
Rosnina, Yusoff ;
Raj, Dana ;
Donny, Yawah ;
Qayyum, Latip ;
Malek, Ab Aziz Abdul .
AQUACULTURE, 2018, 483 :169-172