Integrating network analysis and experimental validation to reveal the mitophagy-associated mechanism of Yiqi Huoxue (YQHX) prescription in the treatment of myocardial ischemia/reperfusion injury

被引：59

作者：

Chen, Mingtai ^{[1
,2
]}

Zhong, Guofu ^{[3
]}

Liu, Mengnan ^{[2
,4
]}

Zhou, Jie ^{[2
]}

Chen, Jianping ^{[6
]}

Zhang, Mingsheng ^{[7
]}

Liu, Qiang ^{[1
]}

Tong, Guangdong ^{[8
]}

Luan, Jienan ^{[1
]}

Zhou, Hua ^{[9
]}

He, Hao ^{[5
]}

机构：

[1] Guangzhou Univ Chinese Med, Shenzhen Tradit Chinese Med Hosp, Dept Cardiovasc Dis, Shenzhen, Peoples R China

[2] Macau Univ Sci & Technol, Fac Chinese Med, State Key Lab Qual Res Chinese Med, Taipa, Macau, Peoples R China

[3] Guangzhou Univ Chinese Med, Shenzhen Tradit Chinese Med Hosp, Intens Care Unit, Shenzhen, Peoples R China

[4] Southwest Med Univ, Dept Cardiovasc, Natl Tradit Chinese Med Clin Res Base, Hosp TCM, Luzhou, Sichuan, Peoples R China

[5] Macau Univ Sci & Technol, Sch Pharm, State Key Lab Qual Res Chinese Med, Taipa, Macau, Peoples R China

[6] Guangzhou Univ Chinese Med, Shenzhen Key Lab Hosp Chinese Med Preparat, Shenzhen Tradit Chinese Med Hosp, Shenzhen, Peoples R China

[7] Guangdong Med Univ, Sch Publ Hlth, Dongguan, Peoples R China

[8] Guangzhou Univ Chinese Med, Shenzhen Tradit Chinese Med Hosp, Dept Liver Dis, Shenzhen, Peoples R China

[9] Guangzhou Univ Chinese Med, Guangdong Prov Hosp Chinese Med, Guangdong Prov Acad Chinese Med Sci, State Key Lab Dampness Syndrome Chinese Med,Affili, Hong Kong, Guangdong, Peoples R China

来源：

PHARMACOLOGICAL RESEARCH | 2023年 / 189卷

基金：

中国国家自然科学基金;

关键词：

Myocardial ischemia/reperfusion injury; Yiqi Huoxue; Mitophagy; Network pharmacology; Traditional Chinese medicine; MITOCHONDRIAL DYSFUNCTION; AUTOPHAGY; DEGRADATION; PROTECTS; BNIP3; CELL; LC3;

D O I：

10.1016/j.phrs.2023.106682

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Myocardial ischemia/reperfusion (I/R) injury is the main cause of increasing postischemic heart failure and currently there is no definite treatment for myocardial I/R injury. It has been suggested that oxidative stressinduced mitochondrial dysfunction plays an important role in the pathological development of myocardial I/R. In this study, Yiqi Huoxue (YQHX) prescription, as a kind of Chinese herbal formula, was developed and shown to alleviate I/R injury. Network analysis combined with ultrahigh-performance liquid chromatography-high resolution mass spectrometry expounded the active components of YQHX and revealed the mitophagyregulation mechanism of YQHX treating I/R injury. In vivo experiments confirmed YQHX significantly alleviated I/R myocardial injury and relieved oxidative stress. In vitro experiments validated that YQHX could relieve hypoxia/reoxygenation injury and attenuate oxidative stress via improving the structure and function of mitochondria, which was strongly related to regulating mitophagy. In summary, this study demonstrated that YQHX, which could alleviate I/R injury via targeting mitophagy, might be a potential therapeutic strategy for myocardial I/R injury.

引用

页数：19

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