Retinoic Acid Signalling in the Pineal Gland Is Conserved across Mammalian Species and Its Transcriptional Activity Is Inhibited by Melatonin

被引:3
作者
Ashton, Anna [1 ]
Clark, Jason [1 ]
Fedo, Julia [1 ]
Sementilli, Angelo [2 ,3 ]
Fragoso, Yara D. D. [1 ,4 ]
McCaffery, Peter [1 ]
机构
[1] Univ Aberdeen, Inst Med Sci, Aberdeen AB25 2ZD, Scotland
[2] Univ Metropolitana Santos, Dept Physiopathol, BR-11050071 Santos, SP, Brazil
[3] Ctr Univ Lusiada, BR-11050071 Santos, SP, Brazil
[4] Univ Metropolitana Santos, Dept Post Grad Studies, BR-11045002 Santos, SP, Brazil
基金
英国生物技术与生命科学研究理事会;
关键词
retinoic acid; RAR; RALDH; Rdh10; cyp26a1; circadian; pineal gland; melatonin; ARYLALKYLAMINE N-ACETYLTRANSFERASE; CLOCK GENES EXPRESSION; VITAMIN-A; MESSENGER-RNA; RECEPTOR-BETA; ROR-ALPHA; ACTIVATION; RHYTHM; IDENTIFICATION; MICE;
D O I
10.3390/cells12020286
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The pineal gland is integral to the circadian timing system due to its role in nightly melatonin production. Retinoic acid (RA) is a potent regulator of gene transcription and has previously been found to exhibit diurnal changes in synthesis and signalling in the rat pineal gland. This study investigated the potential for the interaction of these two systems. PCR was used to study gene expression in mouse and human pineal glands, ex-vivo organotypic cultured rat pineal gland and cell lines. The mouse and human pineal glands were both found to express the necessary components required for RA signalling. RA influences the circadian clock in the brain, therefore the short-term effect of RA on clock gene expression was determined in ex vivo rat pineal glands but was not found to rapidly regulate Per1, Per2, Bmal1, or Cry1. The interaction between RA and melatonin was also investigated and, unexpectedly, melatonin was found to suppress the induction of gene transcription by RA. This study demonstrates that pineal expression of the RA signalling system is conserved across mammalian species. There is no short-term regulation of the circadian clock but an inhibitory effect of melatonin on RA transcriptional activity was demonstrated, suggesting that there may be functional cross-talk between these systems.
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页数:15
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