The inhibition mechanism of bound polyphenols extracted from mung bean coat dietary fiber on porcine pancreatic α-amylase: kinetic, spectroscopic, differential scanning calorimetric and molecular docking

被引：36

作者：

Sun, Nan ^{[1
]}

Xie, Jiayan ^{[1
]}

Zheng, Bing ^{[1
]}

Xie, Jianhua ^{[1
]}

Chen, Yi ^{[1
]}

Hu, Xiaobo ^{[1
]}

Yu, Qiang ^{[1
]}

机构：

[1] Nanchang Univ, State Key Lab Food Sci & Resources, Canada Joint Lab Food Sci & Technol Nanchang, Key Lab Bioact Polysaccharides Jiangxi Prov, 235 Nanjing East Rd, Nanchang 330047, Peoples R China

来源：

FOOD CHEMISTRY | 2024年 / 436卷

基金：

中国国家自然科学基金;

关键词：

Mung bean coat dietary fiber; Bound polyphenols; Porcine pancreatic alpha-amylase; Inhibition mechanism; Molecular docking; ANTIOXIDANT; DIGESTION; PROFILE;

D O I：

10.1016/j.foodchem.2023.137749

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

The inhibitory mechanisms of purified bound polyphenols extracted from mung bean coat dietary fiber (pMBDFBP) on porcine pancreatic alpha-amylase (PPA) were investigated through inhibition kinetics, fluorescence spectroscopy, circular dichroism, differential scanning calorimetry and molecular docking. It was shown that pMBDFBP exerted significant reversible inhibition on PPA in a mixed-type inhibition manner (IC50 = 18.57 +/- 0.30 mu g/ mL), and the combination of the three major components exhibited a synergistic inhibitory effect on PPA. Further, pMBDF-BP bound to the active site or form a polyphenol-enzyme complex at the inactive site through hydrogen bonding and hydrophobic forces, via enhancing the hydrophobicity of the microenvironment surrounding tryptophan and tyrosine residues and promoting the secondary structure of PPA towards a more stable conformation, eventually reducing the enzyme activity. This study provided theoretical evidences for the utilization of bound polyphenols extracted from mung bean coat dietary fiber as a functional component in natural inhibitors of alpha-amylase.

引用

页数：12

共 40 条

[1] Optimizing the extraction of bioactive compounds from pu-erh tea (Camellia sinensis var. assamica) and evaluation of antioxidant, cytotoxic, antimicrobial, antihemolytic, and inhibition of α-amylase and α-glucosidase activities [J].