Characterizing the detection of inactivated Mycoplasma hyopneumoniae DNA in the respiratory tract of pigs

被引：0

作者：

Canturri, Albert ^{[1
,2
]}

Pieters, Maria ^{[1
,2
,3
]}

机构：

[1] Univ Minnesota, Coll Vet Med, Dept Vet Populat Med, St Paul, MN 55455 USA

[2] Univ Minnesota, Coll Vet Med, Vet Diagnost Lab, St Paul, MN 55455 USA

[3] Univ Minnesota, Coll Vet Med, Swine Dis Eradicat Ctr, St Paul, MN 55455 USA

来源：

VETERINARY RESEARCH | 2024年 / 55卷 / 01期

关键词：

Mycoplasma hyopneumoniae; PCR; diagnostics; pigs; DNA; bacterial DNA; QPCR;

D O I：

10.1186/s13567-024-01273-2

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

A positive Mycoplasma hyopneumoniae PCR result in a clinical specimen may eventually represent the mere detection of non-viable bacteria, complicating the diagnostic interpretation. Thus, the objective of this study was to evaluate the PCR detection of non-viable M. hyopneumoniae and its residual cell-free DNA in live pigs. Pigs were inoculated with either active or inactivated M. hyopneumoniae and were sampled for up to 14 days. Mycoplasma hyopneumoniae was not detected by PCR at any timepoint in pigs inoculated with the inactivated bacterium, suggesting that in healthy pigs, the non-viable M. hyopneumoniae DNA was rapidly sensed and cleared.

引用

页数：6

共 21 条

[1] Bacterial DNA on the skin surface overrepresents the viable skin microbiome [J].

Acosta, Ellen M. ;

Little, Katherine A. ;

Bratton, Benjamin P. ;

Lopez, Jaime G. ;

Mao, Xuming ;

Payne, Aimee S. ;

Donia, Mohamed ;

Devenport, Danelle ;

Gitai, Zemer .

ELIFE, 2023, 12

[2] Cell free DNA from respiratory pathogens is detectable in the blood plasma of Cystic Fibrosis patients [J].

Barrett, Sara L. Rassoulian ;

Holmes, Elizabeth A. ;

Long, Dustin R. ;

Shean, Ryan C. ;

Bautista, Gilbert E. ;

Ravishankar, Sumedha ;

Peddu, Vikas ;

Cookson, Brad T. ;

Singh, Pradeep K. ;

Greninger, Alexander L. ;

Salipante, Stephen J. .

SCIENTIFIC REPORTS, 2020, 10 (01)

[3] Analytical and clinical validation of a microbial cell-free DNA sequencing test for infectious disease [J].

Blauwkamp, Timothy A. ;

Thair, Simone ;

Rosen, Michael J. ;

Blair, Lily ;

Lindner, Martin S. ;

Vilfan, Igor D. ;

Kawli, Trupti ;

Christians, Fred C. ;

Venkatasubrahmanyam, Shivkumar ;

Wall, Gregory D. ;

Cheung, Anita ;

Rogers, Zoe N. ;

Meshulam-Simon, Galit ;

Huijse, Liza ;

Balakrishnan, Sanjeev ;

Quinn, James, V ;

Hollemon, Desiree ;

Hong, David K. ;

Vaughn, Marla Lay ;

Kertesz, Mickey ;

Bercovici, Sivan ;

Wilber, Judith C. ;

Yang, Samuel .

NATURE MICROBIOLOGY, 2019, 4 (04) :663-674

[4] MAMMOTH GENOMES SHATTER RECORD FOR OLDEST ANCIENT DNA [J].

Callaway, Ewen .

NATURE, 2021, 590 (7847) :537-+

[5] Pathogenesis of porcine reproductive and respiratory syndrome virus [J].

Chand, Ranjni J. ;

Trible, Benjamin R. ;

Rowland, Raymond R. R. .

CURRENT OPINION IN VIROLOGY, 2012, 2 (03) :256-263

[6] Bluetongue Virus RNA Detection by Real-Time RT-PCR in Post-Vaccination Samples from Cattle [J].

De Leeuw, I. ;

Garigliany, M. ;

Bertels, G. ;

Willems, T. ;

Desmecht, D. ;

De Clercq, K. .

TRANSBOUNDARY AND EMERGING DISEASES, 2015, 62 (02) :157-162

[7] Estimation of the sensitivity of four sampling methods for Mycoplasma hyopneumoniae detection in live pigs using a Bayesian approach [J].

Fablet, C. ;

Marois, C. ;

Kobisch, M. ;

Madec, F. ;

Rose, N. .

VETERINARY MICROBIOLOGY, 2010, 143 (2-4) :238-245

[8] Antibody responses of swine following infection with Mycoplasma hyopneumoniae, M. hyorhinis, M. hyosynoviae and M. flocculare [J].

Gomes Neto, Joao Carlos ;

Strait, Erin L. ;

Raymond, Matthew ;

Ramirez, Alejandro ;

Chris Minion, F. .

VETERINARY MICROBIOLOGY, 2014, 174 (1-2) :163-171

[9] POLYMERASE CHAIN-REACTION DETECTION OF NONVIABLE BACTERIAL PATHOGENS [J].

JOSEPHSON, KL ;

GERBA, CP ;

PEPPER, IL .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1993, 59 (10) :3513-3515

[10] Update on Mycoplasma hyopneumoniae infections in pigs: Knowledge gaps for improved disease control [J].

Maes, D. ;

Sibila, M. ;

Kuhnert, P. ;

Segales, J. ;

Haesebrouck, F. ;

Pieters, M. .

TRANSBOUNDARY AND EMERGING DISEASES, 2018, 65 :110-124

← 1 2 3 →