Reactive oxygen signaling molecule inducible regulation of CRISPR-Cas9 gene editing

被引：0

作者：

Zhao, Jizhong ^{[1
]}

Hu, Hongmei ^{[1
]}

Zhou, Hongling ^{[1
]}

Zhang, Jingwen ^{[1
]}

Wang, Li ^{[2
]}

Wang, Rui ^{[1
,3
]}

机构：

[1] Huazhong Univ Sci & Technol, Sch Pharm, Tongji Med Coll, Hubei Key Lab Nat Med Chem & Resource Evaluat, Wuhan 430030, Peoples R China

[2] Huazhong Univ Sci & Technol, Wuhan Hosp 1, Wuhan 430022, Peoples R China

[3] Shenzhen Huazhong Univ, Sci & Technol Res Inst, Shenzhen 518057, Guangdong, Peoples R China

来源：

CELL BIOLOGY AND TOXICOLOGY | 2023年 / 39卷 / 05期

关键词：

Cas9; Regulation; Boronated; gRNA; ROS; SYSTEM;

D O I：

10.1007/s10565-022-09723-3

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We report development of a controllable gene editing tool that boronated gRNA, simply generated in situ, could regulate binding of gRNA molecules with either Cas9 endonuclease or target genes, thus serving as a modulator that can control CRISPR-Cas9 gene editing. Subsequent treatment with H2O2 facilitates the restoration of gene editing ability of the boronated gRNA to the level of using untreated gRNA. This is one of the few cases using small molecule to regulate CRISPR-Cas9 gene editing, which is a complement to the light approach, displaying great application potential.

引用

页码：2421 / 2429

页数：9

共 26 条

[1] Structural basis of PAM-dependent target DNA recognition by the Cas9 endonuclease
Anders, Carolin
Niewoehner, Ole
Duerst, Alessia
Jinek, Martin
[J]. NATURE, 2014, 513 (7519) : 569 - +
[2] Regulating CRISPR/Cas9 Function through Conditional Guide RNA Control
Brown, Wes
Zhou, Wenyuan
Deiters, Alexander
[J]. CHEMBIOCHEM, 2021, 22 (01) : 63 - 72
[3] Chemically Controlled Epigenome Editing through an Inducible dCas9 System
Chen, Tingjun
Gao, Dan
Zhang, Roushu
Zeng, Guihua
Yan, Hao
Lim, Eunju
Liang, Fu-Sen
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2017, 139 (33) : 11337 - 11340
[4] Versatile Histochemical Approach to Detection of Hydrogen Peroxide in Cells and Tissues Based on Puromycin Staining
Chung, Clive Yik-Sham
Timblin, Greg A.
Saijo, Kaoru
Chang, Christopher J.
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2018, 140 (19) : 6109 - 6121
[5] Davis KM, 2015, NAT CHEM BIOL, V11, P316, DOI [10.1038/NCHEMBIO.1793, 10.1038/nchembio.1793]
[6] Neurodegenerative diseases and oxidative stress
Emerit, J
Edeas, A
Bricaire, F
[J]. BIOMEDICINE & PHARMACOTHERAPY, 2004, 58 (01) : 39 - 46
[7] Gao YC, 2016, NAT METHODS, V13, P1043, DOI [10.1038/NMETH.4042, 10.1038/nmeth.4042]
[8] Reversible RNA acylation for control of CRISPR-Cas9 gene editing
Habibian, Maryam
McKinlay, Colin
Blake, Timothy R.
Kietrys, Anna M.
Waymouth, Robert M.
Wender, Paul A.
Kool, Eric T.
[J]. CHEMICAL SCIENCE, 2020, 11 (04) : 1011 - 1016
[9] Optical Control of CRISPR/Cas9 Gene Editing
Hemphill, James
Borchardt, Erin K.
Brown, Kalyn
Asokan, Aravind
Deiters, Alexander
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2015, 137 (17) : 5642 - 5645
[10] A Bioorthogonal Reaction of N-Oxide and Boron Reagents
Kim, Justin
Bertozzi, Carolyn R.
[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2015, 54 (52) : 15777 - 15781

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