Cellulose nanocrystals-reinforced dual crosslinked double network GelMA/hyaluronic acid injectable nanocomposite cryogels with improved mechanical properties for cartilage tissue regeneration

被引:6
作者
Shariatzadeh, Farinaz Jonidi [1 ]
Solouk, Atefeh [1 ]
Mirzadeh, Hamid [1 ,2 ]
Bonakdar, Shahin [3 ]
Sadeghi, Davoud [1 ]
Khoulenjani, Shadab Bagheri [2 ]
机构
[1] Amirkabir Univ Technol, Tehran Polytech, Biomed Engn Dept, Tehran 1591634311, Iran
[2] Amirkabir Univ Technol, Polymer & Color Engn Dept, Tehran Polytech, Tehran 1591634311, Iran
[3] Pasteur Inst Iran, Natl Cell Bank Dept, Tehran, Iran
关键词
cellulose nanocrystals; double network porous cryogel; GelMA; hyaluronic acid; injectable nanocomposite; HYALURONIC-ACID; GELATIN; HYDROGELS; SCAFFOLDS; PROLIFERATION; CHONDROCYTES; DEGRADATION;
D O I
10.1002/jbm.b.35346
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Improvement of mechanical properties of injectable tissue engineering scaffolds is a current challenge. The objective of the current study is to produce a highly porous injectable scaffold with improved mechanical properties. For this aim, cellulose nanocrystals-reinforced dual crosslinked porous nanocomposite cryogels were prepared using chemically crosslinked methacrylated gelatin (GelMA) and ionically crosslinked hyaluronic acid (HA) through the cryogelation process. The resulting nanocomposites showed highly porous structures with interconnected porosity (>90%) and mean pore size in the range of 130-296 mu m. The prepared nanocomposite containing 3%w/v of GelMA, 20 w/w% of HA, and 1%w/v of CNC showed the highest Young's modulus (10 kPa) and excellent reversibility after 90% compression and could regain its initial shape after injection by a 16-gauge needle in the aqueous media. The in vitro results demonstrated acceptable viability (>90%) and migration of the human chondrocyte cell line (C28/I2), and chondrogenic differentiation of human adipose stem cells. A two-month in vivo assay on a rabbit's ear model confirmed that the regeneration potential of the prepared cryogel is comparable to the natural autologous cartilage graft, suggesting it is a promising alternative for autografts in the treatment of cartilage defects.
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页数:15
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