circRNA mannosidase alpha class 1A member 2 contributes to the proliferation and motility of papillary thyroid cancer cells through upregulating metadherin via absorbing microRNA-449a

被引:4
作者
Feng, Yao [1 ]
Yang, Xinxin [1 ]
Wang, Yibo [2 ]
Chi, Nannan [1 ]
Yu, Jianan [1 ]
Fu, Xiandong [3 ,4 ]
机构
[1] Jiamusi Univ, Sch Clin Med, Jiamusi, Peoples R China
[2] Jiamusi Univ, Dept Med, Jiamusi, Peoples R China
[3] Jiamusi Univ, Dept Gen Surg, Jiamusi, Peoples R China
[4] Jiamusi Univ, Dept Gen Surg, 348 Dexiang St, Jiamusi 154007, Heilongjiang, Peoples R China
关键词
miR-449a; MTDH; papillary thyroid cancer; LYMPH-NODE RATIO; CIRCULAR RNAS; PROGRESSION; CARCINOMA; EXPRESSION; OSTEOSARCOMA; RECURRENCE; INVASION; MIR-449A; SPONGES;
D O I
10.1097/CAD.0000000000001340
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Papillary thyroid carcinoma (PTC) is a common malignancy in endocrine system globally. Accumulating articles have found that circular RNAs (circRNAs) were dysregulated, and they were involved in PTC development. The aim of this project was to explore the function and associated mechanism of circRNA mannosidase alpha class 1A member 2 (circMAN1A2) in PTC progression. The expression of RNA was determined by real-time quantitative PCR. Cell proliferation ability was analyzed by colony formation assay and 5-ethynyl-2 '-deoxyuridine assay. Cell migration and invasion were assessed by wound healing assay and transwell invasion assay, respectively. Protein levels were determined by Western blot assay. Dual-luciferase reporter assay and RNA immunoprecipitation assay were applied to confirm the interaction between microRNA-449a (miR-449a) and circMAN1A2 or metadherin (MTDH). Xenograft tumor model was utilized to explore the effect of circMAN1A2 silencing on tumor growth in vivo. CircMAN1A2 expression was elevated in PTC specimens and three PTC cell lines relative to adjacent normal specimens and Nthy-ori 3-1 cell line. CircMAN1A2 silencing inhibited the proliferation and motility of PTC cells. CircMAN1A2 acted as a molecular sponge of miR-449a, and circMAN1A2 knockdown suppressed PTC development partly through upregulating miR-449a. MiR-449a bound to the 3 ' untranslated region of MTDH, and miR-449a restrained PTC progression partly through down-regulating MTDH. CircMAN1A2 interference suppressed PTC progression in vivo. CircMAN1A2 contributed to the proliferation ability and motility of PTC cells through enhancing MTDH expression via sponging miR-449a.
引用
收藏
页码:44 / 56
页数:13
相关论文
共 38 条
  • [21] Circular RNA circ-NCOR2 accelerates papillary thyroid cancer progression by sponging miR-516a-5p to upregulate metastasis-associated protein 2 expression
    Luan, Sha
    Fu, Peng
    Wang, Xinyu
    Gao, Yue
    Shi, Ke
    Guo, Youmin
    [J]. JOURNAL OF INTERNATIONAL MEDICAL RESEARCH, 2020, 48 (09)
  • [22] miR-199a-5p inhibits the progression of papillary thyroid carcinoma by targeting SNAI1
    Ma, Sugang
    Jia, Wei
    Ni, Shiyu
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2018, 497 (01) : 181 - 186
  • [23] CircRNA: functions and properties of a novel potential biomarker for cancer
    Meng, Shujuan
    Zhou, Hecheng
    Feng, Ziyang
    Xu, Zihao
    Tang, Ying
    Li, Peiyao
    Wu, Minghua
    [J]. MOLECULAR CANCER, 2017, 16
  • [24] SCRAMBLED EXONS
    NIGRO, JM
    CHO, KR
    FEARON, ER
    KERN, SE
    RUPPERT, JM
    OLINER, JD
    KINZLER, KW
    VOGELSTEIN, B
    [J]. CELL, 1991, 64 (03) : 607 - 613
  • [25] Molecular genetics of thyroid cancer: implications for diagnosis, treatment and prognosis
    Nikiforova, Marina N.
    Nikiforov, Yuri E.
    [J]. EXPERT REVIEW OF MOLECULAR DIAGNOSTICS, 2008, 8 (01) : 83 - 95
  • [26] Upregulated circular RNA circ_0025033 promotes papillary thyroid cancer cell proliferation and invasion via sponging miR-1231 and miR-1304
    Pan, Yanhui
    Xu, Tingting
    Liu, Yuanzhi
    Li, Weiwei
    Zhang, Wei
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2019, 510 (02) : 334 - 338
  • [27] Circular RNAs Act as miRNA Sponges
    Panda, Amaresh Chandra
    [J]. CIRCULAR RNAS: BIOGENESIS AND FUNCTIONS, 2018, 1087 : 67 - 79
  • [28] Outcomes of Patients with Differentiated Thyroid Cancer Risk-Stratified According to the American Thyroid Association and Latin American Thyroid Society Risk of Recurrence Classification Systems
    Pitoia, Fabian
    Bueno, Fernanda
    Urciuoli, Carolina
    Abelleira, Erika
    Cross, Graciela
    Tuttle, R. Michael
    [J]. THYROID, 2013, 23 (11) : 1401 - 1407
  • [29] ceRNA in cancer: possible functions and clinical implications
    Qi, Xiaolong
    Zhang, Da-Hong
    Wu, Nan
    Xiao, Jun-Hua
    Wang, Xiang
    Ma, Wang
    [J]. JOURNAL OF MEDICAL GENETICS, 2015, 52 (10) : 710 - 718
  • [30] Lymph Node Ratio Predicts Recurrence in Papillary Thyroid Cancer
    Schneider, David F.
    Mazeh, Haggi
    Chen, Herbert
    Sippel, Rebecca S.
    [J]. ONCOLOGIST, 2013, 18 (02) : 157 - 162