Advances in Biosynthesis of Non-Canonical Amino Acids (ncAAs) and the Methods of ncAAs Incorporation into Proteins

被引：14

作者：

Chen, Liang ^{[1
]}

Xin, Xiulan ^{[1
]}

Zhang, Yuning ^{[1
]}

Li, Shunyao ^{[1
]}

Zhao, Xiaoman ^{[1
]}

Li, Song ^{[1
]}

Xu, Zhaochu ^{[1
]}

机构：

[1] Beijing Polytech, Coll Bioengn, Beijing 100176, Peoples R China

来源：

MOLECULES | 2023年 / 28卷 / 18期

关键词：

non-canonical amino acid; genetic code expansion; biosynthesis; incorporation; tailor-made protein; GAMMA-AMINOBUTYRIC-ACID; IN-VIVO INCORPORATION; ESCHERICHIA-COLI; L-HOMOSERINE; IMMUNOCHEMICAL TERMINATION; GENETIC INCORPORATION; MICROBIAL-PRODUCTION; CODON; 5-HYDROXYTRYPTOPHAN; DERIVATIVES;

D O I：

10.3390/molecules28186745

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The functional pool of canonical amino acids (cAAs) has been enriched through the emergence of non-canonical amino acids (ncAAs). NcAAs play a crucial role in the production of various pharmaceuticals. The biosynthesis of ncAAs has emerged as an alternative to traditional chemical synthesis due to its environmental friendliness and high efficiency. The breakthrough genetic code expansion (GCE) technique developed in recent years has allowed the incorporation of ncAAs into target proteins, giving them special functions and biological activities. The biosynthesis of ncAAs and their incorporation into target proteins within a single microbe has become an enticing application of such molecules. Based on that, in this study, we first review the biosynthesis methods for ncAAs and analyze the difficulties related to biosynthesis. We then summarize the GCE methods and analyze their advantages and disadvantages. Further, we review the application progress of ncAAs and anticipate the challenges and future development directions of ncAAs.

引用

页数：19

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