Diverse biological activities and secondary metabolites profile of Penicillium brevicompactum HE19ct isolated from the high-Andean medicinal plant Perezia coerulescens

被引：6

作者：

Tamariz-Angeles, Carmen ^{[1
]}

Olivera-Gonzales, Percy ^{[1
]}

Santillan-Torres, Miguelina ^{[1
]}

Briceno-Luna, Veronica ^{[2
]}

Silva-Villafana, Alex ^{[3
]}

Villena, Gretty K. ^{[4
]}

机构：

[1] Univ Nacl Santiago Antunez Mayolo, Ctr Invest Biodivers & Recursos Genet, Av Centenario 200, Huaraz 02002, Peru

[2] Univ Nacl Santiago Antunez Mayolo, Lab Quim, Av Centenario 200, Huaraz 02002, Peru

[3] Univ Nacl Santiago Antunez Mayolo, Av Centenario 200,Independencia, Huaraz 02002, Ancash, Peru

[4] Univ Nacl Agr La Molina, Lab Micol & Biotecnol, Av La Molina S-N,Lima 12, Lima, Peru

来源：

FUNGAL BIOLOGY | 2023年 / 127卷 / 12期

关键词：

Penicillium; Endophyte fungi; Mountain; Cordillera blanca; Bioactive metabolites; ENDOPHYTIC FUNGI; IN-VITRO; ANTIOXIDANT; ANTIBACTERIAL; COMMUNITIES; CULTIVARS; ANCASH; GENES;

D O I：

10.1016/j.funbio.2023.10.002

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Endophytic fungi produced attractive primary and secondary metabolites for industries, pharmacology, and biotechnology. The bioactive potential of HE19ct, identified as Penicillium brevicompactum according to ITS-BenA-caM, was addressed. Antimicrobial and antioxidant activities and secondary metabolite contents using four culture media in Agar-plate (ApF) and Submerged (SmF) fermentation were evaluated. Some plant growth -promoting (PGP) traits and their related genes were tested. HE19ct exhibited antimicrobial activity against Staphylococcus aureus, Enterococcus faecalis, Candida albicans, C. tropicalis, Fusarium sp., Geotrichum candidum, and Alternaria sp. All cultures showed DPPH scavenging activity and phenolic compounds, where ethyl acetate extract of SmF with malt extract showed higher activity and SmF/ApF with potato-dextrose exhibited higher yield, respectively. HE19ct solubilized tricalcium-phosphate and produced siderophore, endoglucanase, pro-teinase, and amylase. It enhanced the alfalfa's germination at 15 degrees C, root development, and phenols production at 15 and 24 degrees C. Phenols, tannins, anthraquinones, triterpenoids/steroids, and alkaloids production were detected depending on culture media. Polyketide synthase type I gene (PksI), subtilisin-like protease prb 1 (Pbr), and siderophore D (sidD) were PCR-amplified. Finally, HE19CT could be a promising source of interesting bioactive compounds for pharmacology and agriculture mainly in extreme conditions, then metabolomic and functional genetic research must be performed to support their appropriate application.

引用

页码：1439 / 1450

页数：12

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