A high-throughput fully automatic biosensing platform for efficient COVID-19 detection

被引：12

作者：

Rong, Guoguang ^{[1
,2
,3
]}

Zheng, Yuqiao ^{[1
,2
,3
]}

Li, Xiangqing ^{[2
,3
]}

Guo, Mengzhun ^{[4
,5
,6
]}

Su, Yi ^{[1
,2
,3
]}

Bian, Sumin ^{[1
,2
,3
]}

Dang, Bobo ^{[4
,5
,6
]}

Chen, Yin ^{[7
]}

Zhang, Yanjun ^{[7
]}

Shen, Linhai ^{[8
]}

Jin, Hui ^{[8
]}

Yan, Renhong ^{[4
,5
]}

Wen, Liaoyong ^{[2
,3
]}

Zhu, Peixi ^{[9
]}

Sawan, Mohamad ^{[1
,2
,3
]}

机构：

[1] Westlake Univ, Sch Engn, CenBRAIN Neurotech, 600 Dunyu Rd, Hangzhou 310030, Zhejiang, Peoples R China

[2] Westlake Univ, Sch Engn, 600 Dunyu Rd, Hangzhou 310030, Zhejiang, Peoples R China

[3] Westlake Inst Adv Study, Inst Adv Study, Hangzhou 310024, Zhejiang, Peoples R China

[4] Westlake Univ, Sch Life Sci, Key Lab Struct Biol Zhejiang Prov, Hangzhou 310030, Zhejiang, Peoples R China

[5] Westlake Lab Life Sci & Biomed, Ctr Infect Dis Res, Hangzhou 310030, Zhejiang, Peoples R China

[6] Westlake Inst Adv Study, Inst Biol, Hangzhou 310030, Zhejiang, Peoples R China

[7] Zhejiang Prov Ctr Dis Control & Prevent, 3399 Binsheng Rd, Hangzhou 310051, Zhejiang, Peoples R China

[8] Hangzhou Ctr Dis Control & Prevent, 568 Mingshi Rd, Hangzhou 310021, Zhejiang, Peoples R China

[9] Zhejiang Univ Technol, Coll Pharmaceut Sci, Hangzhou 310014, Zhejiang, Peoples R China

来源：

BIOSENSORS & BIOELECTRONICS | 2023年 / 220卷

关键词：

Biosensor; Localized surface plasmon resonance (LSPR); SARS-CoV-2; COVID-19; Automatic detection; High throughput; SURFACE-PLASMON RESONANCE; DIAGNOSIS; SARS-COV-2; SIZE;

D O I：

10.1016/j.bios.2022.114861

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

We propose a label-free biosensor based on a porous silicon resonant microcavity and localized surface plasmon resonance. The biosensor detects SARS-CoV-2 antigen based on engineered trimeric angiotensin converting enzyme-2 binding protein, which is conserved across different variants. Robotic arms run the detection process including sample loading, incubation, sensor surface rinsing, and optical measurements using a portable spec-trometer. Both the biosensor and the optical measurement system are readily scalable to accommodate testing a wide range of sample numbers. The limit of detection is 100 TCID50/ml. The detection time is 5 min, and the throughput of one single robotic site is up to 384 specimens in 30 min. The measurement interface requires little training, has standard operation, and therefore is suitable for widespread use in rapid and onsite COVID-19 screening or surveillance.

引用

页数：9

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