Development of a microencapsulated probiotic containing Pediococcus acidilactici WU222001 against avian pathogenic Escherichia coli

被引:3
作者
Mitsuwan, Watcharapong [1 ,2 ,3 ]
Saengsawang, Phirabhat [1 ,2 ]
Jeenkeawpieam, Juthatip [1 ,2 ]
Nissapatorn, Veeranoot [4 ,5 ]
Pereira, Maria de Lourdes [6 ,7 ]
Kitpipit, Warangkana [1 ,2 ,8 ]
Thomrongsuwannakij, Thotsapol [1 ,2 ]
Poothong, Saranporn [9 ]
Vimon, Sasi [1 ,2 ]
机构
[1] Walailak Univ, Akkhraratchakumari Vet Coll, Nakhon Si Thammarat 80160, Thailand
[2] Walailak Univ, One Hlth Res Ctr, Nakhon Si Thammarat 80160, Thailand
[3] Walailak Univ, Ctr Excellence Innovat Essential Oil & Bioact Cpds, Nakhon Si Thammarat 80160, Thailand
[4] Walailak Univ, Sch Allied Hlth Sci, Southeast Asia Water Team, World Union Herbal Drug Discovery, Nakhon Si Thammarat, Thailand
[5] Walailak Univ, Res Excellence Ctr Innovat & Hlth Prod, Nakhon Si Thammarat, Thailand
[6] Univ Aveiro, Aveiro Inst Mat, CICECO, P-3010193 Aveiro, Portugal
[7] Univ Aveiro, Dept Med Sci, P-3010193 Aveiro, Portugal
[8] Walailak Univ, Food Technol & Innovat Ctr Excellence, Nakhon Si Thammarat 80160, Thailand
[9] Chulalongkorn Univ, Fac Vet Sci, Dept Anim Husb, Bangkok 10330, Thailand
关键词
antibacterial activity; avian pathogenic Escherichia coli; microcapsule; microencapsulation; Pediococcus acidilactici; probiotics; VIABILITY;
D O I
10.14202/vetworld.2023.1131-1140
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
Background and Aim: Probiotics are beneficial microorganisms for humans and animals. In this study, we developed a microencapsulated probiotic with antibacterial activity against avian pathogenic Escherichia coli (APEC). Materials and Methods: Alignment of the 16S rRNA sequences of the isolate WU222001 with those deposited in GenBank revealed that the isolate was Pediococcus acidilactici with 99.6% homology. This bacterium was characterized as a probiotic based on its tolerance toward in vitro gastrointestinal tract (GIT) conditions, hydrophobicity, and auto-aggregation. The antibacterial activity of the probiotic's culture supernatant against APEC was investigated using a broth microdilution assay. Pediococcus acidilactici was microencapsulated using sodium alginate and agar with diameters ranging from 47 to 61 mu m. Then, physicochemical characteristics and stability of the microcapsules were determined. Results: The isolate was characterized as a probiotic based on its resistance to low pH, bile salts, and pancreatin, with relative values of 79.2%, 70.95%, and 90.64%, respectively. Furthermore, the bacterium exhibited 79.56% auto-aggregation and 55.25% hydrophobicity at 24 h. The probiotic's culture supernatant exhibited strong antibacterial activity against clinical APEC isolates with minimum inhibitory concentration and minimum bactericidal concentration of 12.5% and 25% v/v, respectively. Microencapsulation-enhanced bacterial viability in GIT compared to free cells. Moreover, 89.65% of the encapsulated cells were released into the simulated intestinal fluid within 4 h. The viable count in microcapsules was 63.19% after 3 months of storage at 4 degrees C. Conclusion: The results indicated that the culture supernatant of P. acidilactici inhibited the growth of APEC. In addition, microencapsulation extends the viability of P. acidilactici under harsh conditions, indicating its potential application in the feed production.
引用
收藏
页码:1131 / 1140
页数:10
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