Evaluation of the effects of glucose concentration in extender and sperm concentration in straw for cryopreservation of Atlantic sturgeon (Acipenser oxyrinchus) semen

被引：3
作者：
Judycka, Sylwia [1 ]
Dietrich, Mariola A. [1 ]
Szczepkowska, Bo zena [2 ]
Szczepkowski, Miros law [2 ]
Liszewska, Ewa [1 ]
Ciereszko, Andrzej [1 ]
机构：
[1] Polish Acad Sci, Inst Anim Reprod & Food Res, Dept Gametes & Embryo Biol, Tuwima 10, PL-10748 Olsztyn, Poland
[2] Natl Inland Fisheries Res Inst Olsztyn, Dept Sturgeon Fish Breeding, Pieczarki, Poland
来源：
AQUACULTURE REPORTS | 2024年 / 35卷
关键词：
Sperm motility parameters; Sturgeon; Cryopreservation; Sperm concentration; FERTILIZING-CAPACITY; RUTHENUS; MOTILITY; SPERMATOZOA; MANAGEMENT; MORPHOLOGY; ABILITY; BAERI;
D O I：
10.1016/j.aqrep.2024.101995
中图分类号：
S9 [水产、渔业];
学科分类号：
0908 ;
摘要：
The Atlantic sturgeon (Acipenser oxyrinchus) faces unique conservation challenges due to its extended maturation period and irregular spawning patterns, which restrict its reproductive capacity. Therefore, in order to overcome these difficulties we have made a first attempt to develop effective cryopreservation procedure for Atlantic sturgeon through evaluation of the effects of the glucose concentration in extender and final sperm concentration in straw on sperm motility parameters. A remarkably high (81 +/- 8%) postthaw sperm motility was obtained. The other sperm velocity and trajectory parameters were differentially affected both by concentrations of glucose or sperm and cryopreservation process. Our results undoubtedly show that the post-thaw sperm motility of Atlantic sturgeon is highly influenced by the final glucose and sperm concentration. The recommended cryopreservation procedure includes 0.05 M glucose and 7.5% methanol at 1.0x109 spermatozoa ml- 1. We have confirmed that our elaborated protocol can be used for cryopreservation of Atlantic sturgeon semen which can be subsequently used for long-term conservation of this endangered species, offering a valuable tool in efforts to safeguard its genetic diversity and contribute to its survival.
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页数：7
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