Human liver organoids: From generation to applications

被引：14

作者：

Afonso, Marta B. ^{[1
]}

Marques, Vanda ^{[1
]}

van Mil, Saskia W. C. ^{[2
,3
]}

Rodrigues, Cecilia M. P. ^{[1
]}

机构：

[1] Univ Lisbon, Res Inst Med IMed ULisboa, Fac Pharm, Lisbon, Portugal

[2] Univ Med Ctr Utrecht, Ctr Mol Med, Utrecht, Netherlands

[3] Univ Utrecht, Utrecht, Netherlands

来源：

HEPATOLOGY | 2024年 / 79卷 / 06期

关键词：

PLURIPOTENT STEM-CELLS; DIRECTED DIFFERENTIATION; HUMAN CHOLANGIOCYTES; STORAGE-DISEASE; WILSONS-DISEASE; GENE CORRECTION; MOUSE MODEL; DEFICIENCY; HEPATOCYTES; EXPANSION;

D O I：

10.1097/HEP.0000000000000343

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

In the last decade, research into human hepatology has been revolutionized by the development of mini human livers in a dish. These liver organoids are formed by self-organizing stem cells and resemble their native counterparts in cellular content, multicellular architecture, and functional features. Liver organoids can be derived from the liver tissue or pluripotent stem cells generated from a skin biopsy, blood cells, or renal epithelial cells present in urine. With the development of liver organoids, a large part of previous hurdles in modeling the human liver is likely to be solved, enabling possibilities to better model liver disease, improve (personalized) drug testing, and advance bioengineering options. In this review, we address strategies to generate and use organoids in human liver disease modeling, followed by a discussion of their potential application in drug development and therapeutics, as well as their strengths and limitations.

引用

页码：1432 / 1451

页数：20

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