Bartonella spp. Infections Identified by Molecular Methods, United States

被引：3

作者：

McCormick, David W. ^{[1
]}

Rassoulian-Barrett, Sara L. ^{[2
]}

Hoogestraat, Daniel R. ^{[2
]}

Salipante, Stephen J. ^{[2
]}

SenGupta, Dhruba ^{[2
]}

Dietrich, Elizabeth A. ^{[1
]}

Cookson, Brad T. ^{[2
]}

Marx, Grace E. ^{[1
]}

Lieberman, Joshua A. ^{[2
]}

机构：

[1] Ctr Dis Control & Prevent, 3156 Rampart Rd, Ft Collins, CO 80521 USA

[2] Univ Washington, Seattle, WA USA

来源：

EMERGING INFECTIOUS DISEASES | 2023年 / 29卷 / 03期

关键词：

CAT-SCRATCH DISEASE; ENDOCARDITIS; HENSELAE; CULTURE; MANIFESTATIONS; CLARRIDGEIAE; PREVALENCE; ANTIBODIES; QUINTANA; CHILDREN;

D O I：

10.3201/eid2902.221223

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Molecular methods can enable rapid identification of Bartonella spp. infections, which are difficult to diagnose by using culture or serology. We analyzed clinical test results of PCR that targeted bacterial 16S rRNA hypervariable V1-V2 regions only or in parallel with PCR of Bartonellaspecific ribC gene. We identified 430 clinical specimens infected with Bartonella spp. from 420 patients in the United States. Median patient age was 37 (range 1-79) years; 62% were male. We identified B. henselae in 77%, B. quintana in 13%, B. clarridgeiae in 1%, B. vinsonii in 1%, and B. washoensis in 1% of specimens. Eighty-three percent of specimens with B. quintana were cardiac specimens; 34% of specimens with B. henselae were lymph nodes. We detected novel or uncommon Bartonella spp. in 9 patients. Molecular diagnostic testing can identify Bartonella spp. infections, including uncommon and undescribed species, and might be particularly useful for patients who have culture-negative endocarditis or lymphadenitis.

引用

页码：467 / 476

页数：10

共 36 条

[1]

ALTSCHUL SF, 1990, J MOL BIOL, V215, P403, DOI 10.1006/jmbi.1990.9999

[2]

[Anonymous], 2020, The R Project for Statistical Computing

[3] Bartonella species as a cause of infective endocarditis in the UK [J].

Chaloner, G. L. ;

Harrison, T. G. ;

Birtles, R. J. .

EPIDEMIOLOGY AND INFECTION, 2013, 141 (04) :841-846

[4] Human Bartonellosis: An Underappreciated Public Health Problem? [J].

Cheslock, Mercedes A. ;

Embers, Monica E. .

TROPICAL MEDICINE AND INFECTIOUS DISEASE, 2019, 4 (02)

[5] Comprehensive evaluation of complex polymicrobial specimens using next generation sequencing and standard microbiological culture [J].

Cummings, Lisa A. ;

Hoogestraat, Daniel R. ;

Rassoulian-Barrett, Sara L. ;

Rosenthal, Christopher A. ;

Salipante, Stephen J. ;

Cookson, Brad T. ;

Hoffman, Noah G. .

SCIENTIFIC REPORTS, 2020, 10 (01)

[6] Clinical Next Generation Sequencing Outperforms Standard Microbiological Culture for Characterizing Polymicrobial Samples [J].

Cummings, Lisa A. ;

Kurosawa, Kyoko ;

Hoogestraat, Daniel R. ;

SenGupta, Dhruba J. ;

Candra, Febrian ;

Doyle, Michael ;

Thielges, Stephanie ;

Land, Tyler A. ;

Rosenthal, Christopher A. ;

Hoffman, Noah G. ;

Salipante, Stephen J. ;

Cookson, Brad T. .

CLINICAL CHEMISTRY, 2016, 62 (11) :1465-1473

[7] Identification of an Emergent Pathogen, Bartonella vinsonii, Using Next-Generation Sequencing in a Patient With Culture-Negative Endocarditis [J].

Downey, Rachel D. ;

Russo, Susan M. ;

Hauger, Sarmistha B. ;

Murphey, Donald K. ;

Marx, Grace ;

Huynh, Thanhthao ;

Denison, Amy M. ;

Quirt, Rachel ;

Bailey, Ann ;

Fernandez, Marisol .

JOURNAL OF THE PEDIATRIC INFECTIOUS DISEASES SOCIETY, 2021, 10 (02) :213-216

[8] False Negative Results in Bartonellosis Diagnosis [J].

Drummond, Marina Rovani ;

dos Santos, Luciene Silva ;

da Silva, Marilene Neves ;

de Almeida, Amanda Roberta ;

Diniz, Pedro Paulo Vissotto de Paiva ;

Angerami, Rodrigo ;

Neves Ferreira Velho, Paulo Eduardo .

VECTOR-BORNE AND ZOONOTIC DISEASES, 2019, 19 (06) :453-454

[9] Bartonella, a Common Cause of Endocarditis: a Report on 106 Cases and Review [J].

Edouard, Sophie ;

Nabet, Cecile ;

Lepidi, Hubert ;

Fournier, Pierre-Edouard ;

Raoult, Didier .

JOURNAL OF CLINICAL MICROBIOLOGY, 2015, 53 (03) :824-829

[10] Beyond cat scratch disease:: Widening spectrum of Bartonella henselae infection [J].

Florin, Todd A. ;

Zaoutis, Theoklis E. ;

Zaoutis, Lisa B. .

PEDIATRICS, 2008, 121 (05) :E1413-E1425

← 1 2 3 4 →