Protocol to study projection-specific circuits in the basal ganglia of adult mice using viral vector tracing, optogenetics, and patch-clamp technique

被引：1

作者：

Ji, Ya-Wei ^{[1
,2
,3
]}

Xu, Xiang-Ying ^{[1
,2
,3
]}

Yin, Cui ^{[1
,2
,3
]}

Zhou, Chunyi ^{[1
,2
,3
]}

Xiao, Cheng ^{[1
,2
,3
]}

机构：

[1] Xuzhou Med Univ, Sch Anesthesiol, Xuzhou 221004, Jiangsu, Peoples R China

[2] Xuzhou Med Univ, Jiangsu Prov Key Lab Anesthesiol, Xuzhou 221004, Jiangsu, Peoples R China

[3] Xuzhou Med Univ, Sch Anesthesiol, NMPA Key Lab Res & Evaluat Narcot & Psychotrop Dru, Xuzhou 221004, Jiangsu, Peoples R China

来源：

STAR PROTOCOLS | 2023年 / 4卷 / 03期

基金：

中国国家自然科学基金;

关键词：

SUBTHALAMIC NUCLEUS; SUBSTANTIA-NIGRA; NEURONS;

D O I：

10.1016/j.xpro.2023.102551

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Analysis of synaptic strength and plasticity provides functional insights of complicated neural circuits. Here, we describe steps for cell-and projection-specific optogenetic manipulation of divergent basal ganglia circuits using anterograde and retrograde viral vectors. We quantitatively analyze synaptic function of these circuits utilizing a patch-clamp technique. This protocol is applicable to probe potential circuit targets for treatment of brain diseases. For complete details on the use and execution of this protocol, please refer to Ji et al.1

引用

页数：22

共 24 条

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